A novel chemiluminescence reaction system for the determination of lincomycin with diperiodatonickelate(IV)

A sensitive and high selective chemiluminescence (CL) method was developed for the determination of lincomycin in acid medium using diperiodatonickelate as a reagent. The mechanism leading to luminescence is discussed by comparing the spectra of fluorescence and CL. Relative CL intensity is linear in the range from 8.0 ng mL^?1 to 1.0 µg mL^?1, the limit of detection is 2.5 ng mL^?1 (3σ), and the relative standard deviation is 4.0% at 0.1 µg mL^?1 of lincomycin (n?=?7). The method was successfully applied to the determination of lincomycin in injections, human urine, and in serum samples.