Sandwich-type amperometric immunosensor for human immunoglobulin G using antibody-adsorbed Au/SiO2 nanoparticles |
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| Authors: | Lingling Wang Xueen Jia Yaping Zhou Qingji Xie Shouzhuo Yao |
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| Institution: | 1. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China
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| Abstract: | A novel sandwich-type electrochemical immunosensor for human immunoglobulin G (hIgG) was developed using Au/SiO2 nanoparticles (NPs) with adsorbed horseradish peroxidase-anti-hIgG as the secondary antibody layer. The signal readout is based on the amperometric response to the catalytic reduction of hydrogen peroxide at an AuNPs-polythionine modified glassy carbon electrode. Under optimized conditions, the linear range is from 0.1 to 200 ng·mL?1, with a detection limit of 0.035 ng·mL?1 (at an S/N of 3). The immunosensor exhibited a performance that is better than that based on Au/SiO2NPs-excluded secondary antibody. |
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