Radioiodination
of insulin using N-succinimidyl 5-(tributylstannyl)-3-pyridine-carboxylate
(SPC) as a bi-functional linker: Synthesis and biodistribution in mice</p>
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Authors: | Yuanyou Yang Ning Liu Liangbiao Zan Jiali Liao Jiannan Jin |
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Institution: | (1) Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, P.R. China;(2) Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, P.R. China;(3) Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, P.R. China;(4) Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, P.R. China;(5) Key Laboratory of Radiation Physics and Technology (Sichuan University), Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, P.R. China |
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Abstract: | Summary Insulin receptors are overexpressed on a number of
human tumors, leading to significant affinity of insulin to these tumors. It
is appealing to receptor-targeted radiotherapy for malignant tumors if insulin
is labeled with suitable radionuclide. In this paper, N-succinimidyl
5-(tributylstannyl)-3-pyridinecarboxylate (SPC), a potential bi-functional
linker for radioiodination of proteins or peptides, was synthesizedby using 5-bromonicotinic acid as the
starting material. Then, with this bi-functional linker, insulin was conjugated
with 131I, and the tissue distribution of the labeled insulin (131I-SIPC-insulin) in normal mice was
investigated. The results showed that insulin </span> could be
conjugated with131I using SPC as the linker </span> in a
labeling yield of40-58%, and with radiochemical purity of more than 98%
after purification bySephadex?G-10. Even kept at room temperature for
72 hours, the radiochemical purity of 131I-SIPC-insulin was still more than 97%, implying
that the conjugated insulin was constantly stable in vitro.Meanwhile, in order to evaluate the in
vivo stability of the conjugated compounds, insulin was also labeled with 131I
by a direct method using chloramine-T (Ch-T) as the electrophilic agents.Biodistribution of131I-SIPC-insulinin
micesuggested that 131I could clear rapidly from the blood,mainly excreted by kidney. However, 131I
uptake of mice with131I-SIPC-insulin
in some key organs, especially in thyroid and stomach, were much less (150 or
30 times) than that with the direct labeled insulin (131I-insulin). Additionally, it was noted
that 131I-SIPC-insulin
hasmuch betterinvivo stability than131I-insulin.</p>
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Keywords: | |
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