Composite Fiber Spun Mat Synthesis and In Vitro Biocompatibility for Guide Tissue Engineering |
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Authors: | Rodrigo Osorio-Arciniega Manuel García-Hiplito Octavio Alvarez-Fregoso Marco Antonio Alvarez-Perez |
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Institution: | 1.Laboratorio de Bioingeniería de Tejidos, DEPeI, Facultad de Odontología, Universidad Nacional Autónoma de México, Circuito Exterior s/n. Cd. Universitaria, Coyoacán 04510, Mexico;2.Instituto de Investigaciones en Materiales, Circuito Exterior s/n. Cd. Universitaria, Coyoacán 04510, Mexico; (M.G.-H.); (O.A.-F.) |
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Abstract: | Composite scaffolds are commonly used strategies and materials employed to achieve similar analogs of bone tissue. This study aims to fabricate 10% wt polylactic acid (PLA) composite fiber scaffolds by the air-jet spinning technique (AJS) doped with 0.5 or 0.1 g of zirconium oxide nanoparticles (ZrO2) for guide bone tissue engineering. ZrO2 nanoparticles were obtained by the hydrothermal method and characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). SEM and fourier-transform infrared spectroscopy (FTIR) analyzed the synthesized PLA/ZrO2 fiber scaffolds. The in vitro biocompatibility and bioactivity of the PLA/ZrO2 were studied using human fetal osteoblast cells. Our results showed that the hydrothermal technique allowed ZrO2 nanoparticles to be obtained. SEM analysis showed that PLA/ZrO2 composite has a fiber diameter of 395 nm, and the FITR spectra confirmed that the scaffolds’ chemical characteristics are not affected by the synthesized technique. In vitro studies demonstrated that PLA/ZrO2 scaffolds increased cell adhesion, cellular proliferation, and biomineralization of osteoblasts. In conclusion, the PLA/ZrO2 scaffolds are bioactive, improve osteoblasts behavior, and can be used in tissue bone engineering applications. |
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Keywords: | composite scaffold guide tissue engineering polylactic acid (PLA) nanofibers zirconium ceramic biocompatibility |
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