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Differential dynamic microscopy of bacterial motility
Authors:Wilson L G  Martinez V A  Schwarz-Linek J  Tailleur J  Bryant G  Pusey P N  Poon W C K
Affiliation:SUPA and COSMIC, School of Physics & Astronomy, The University of Edinburgh, Mayfield Road, Edinburgh EH9 3JZ, United Kingdom.
Abstract:We demonstrate a method for the fast, high-throughput characterization of the dynamics of active particles. Specifically, we measure the swimming speed distribution and motile cell fraction in Escherichia coli suspensions. By averaging over ~10(4) cells, our method is highly accurate compared to conventional tracking, yielding a routine tool for motility characterization. We find that the diffusivity of nonmotile cells is enhanced in proportion to the concentration of motile cells.
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