| 荧光光谱法研究氟咯沙星-锌(Ⅱ)-牛血清白蛋白的三元配合物 |
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| 引用本文： | 吴根华,汪婕,郭畅,王冬香,赵婷婷.荧光光谱法研究氟咯沙星-锌(Ⅱ)-牛血清白蛋白的三元配合物[J].光谱学与光谱分析,2007,27(4):765-768. |
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| 作者姓名： | 吴根华 汪婕 郭畅 王冬香 赵婷婷 |
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| 作者单位： | 安庆师范学院化学与环境科学学院,安徽,安庆,246011 |
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| 摘 要： | 在模拟生理条件下,用荧光光谱法研究了氟咯沙星对牛血清白蛋白,锌(Ⅱ)对牛血清白蛋白以及锌(Ⅱ)对氟咯沙星和牛血清白蛋白荧光光谱特性的影响.锌(Ⅱ)和氟咯沙星均可使牛血清白蛋白的荧光强度发生猝灭,但是在锌(Ⅱ)存在下,氟咯沙星对牛血清白蛋白的荧光猝灭作用显著增强.根据荧光猝灭双倒数图计算氟咯沙星和牛血清白蛋白之间的结合常数5.44×104,结合位点数是1.05;锌(Ⅱ)和牛血清白蛋白之间的结合常数是2.19×109,结合位点数是2.
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| 关 键 词： | 荧光猝灭光谱 氟咯沙(FLRX) 锌(Ⅱ) 牛血清白蛋白(BSA) 二元配合物 三元配合物 |
| 文章编号： | 1000-0593（2007）04-0765-04 |
| 收稿时间： | 2006-01-09 |
| 修稿时间： | 2006-05-28 |
Study on the Ternary Complex of FLRX,Zinc(Ⅱ) and BSA by the Method of Fluorescence |
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| WU Gen-hua,WANG Jie,GUO Chang,WANG Dong-xiang,ZHAO Ting-ting.Study on the Ternary Complex of FLRX,Zinc(Ⅱ) and BSA by the Method of Fluorescence[J].Spectroscopy and Spectral Analysis,2007,27(4):765-768. |
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| Authors: | WU Gen-hua WANG Jie GUO Chang WANG Dong-xiang ZHAO Ting-ting |
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| Institution: | Department of Chemistry, Anqing Normal College, Anqing 246011, China |
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| Abstract: | The influences of fleroxacin (FLRX) on the fluorescence of bovine serum albumin(BSA), zinc(II) on that of bovine serum albumin, and zinc(II) on the of fleroxacin and bovine serum albumin were studied under imitated the physiological condition. It was shown that both fleroxacin and zinc(II) have a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism. But the fluorescence quenching action of fleroxacin on BSA was much stronger in the presence of zinc (II). The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal equation, and the binding constant(K) and the binding sites(n) were obtained. In the system of binary complex of FLRX and BSA, K = 5.44 x 10(4) and n = 1.05, while in the system of binary complex of zinc(II) and BSA, K = 2.19 x 10(9) and n = 2. |
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| Keywords: | Fluorescence spectrum Fleroxacin Zinc ions Bovine serum albumin Binary complex Ternary complex |
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