| 抗氧化剂抗脂质过氧化机制的ESR研究 |
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| 引用本文： | 高姝娟,赵光程.抗氧化剂抗脂质过氧化机制的ESR研究[J].波谱学杂志,1998,15(2):139-144. |
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| 作者姓名： | 高姝娟 赵光程 |
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| 作者单位： | 1.吉林大学酶工程实验室, 长春 130023;2.东北师范大学生命科学学院, 长春 130024;3.吉林大学超分子结构与光谱实验室, 长春 130023;4.白求恩医科大学第一临床学院, 长春 130021 |
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| 基金项目： | 吉林大学酶工程实验室开放课题资助 |
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| 摘 要： | 以NADH诱导的心肌线粒体损伤体系为模型,在体外用ESR研究了谷胱甘肽(GSH)、超氧化物歧化酶(SOD)及辣根过氧化物酶(HRP)的抗氧化机制.结果表明,低浓度的GSH可部分抑制体系中自由基的生成;SOD与HRP以适当比例共同作用,可完全抑制体系中自由基的生成,较好地防止脂质过氧化的发生.
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| 关 键 词： | ESR 自旋捕捉 抗氧化剂 线粒体 |
| 收稿时间： | 1997-09-01 |
ESR STUDY ON THE MECHANISM OF ANTI LIPID PEROXIDATION OF ANTIOXIDANTS |
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| Gao Shujuan,Liu Dongbo ,Luo Guimin,Yang Tongshu and Shen Jiacong.ESR STUDY ON THE MECHANISM OF ANTI LIPID PEROXIDATION OF ANTIOXIDANTS[J].Chinese Journal of Magnetic Resonance,1998,15(2):139-144. |
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| Authors: | Gao Shujuan Liu Dongbo Luo Guimin Yang Tongshu and Shen Jiacong |
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| Institution: | 1.Key Lab of Enzyme Engineering, Jilin University, Changchun, 130023;2.Northeast Normal University, Changchun, 130024;3.Key Lab of Supernolecular Structure and Spectrum, Jilin University, Changchun, 130023;4.The First Teaching Hospital of Norman Bethune University of Medical Science, Changchun, 130021 |
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| Abstract: | Experiments were carried out using ESR spectra with 5,5 dimethyl 1 pyrroline N oxide (DMPO) as spin trap to study the antioxidant mechanism of glutathione (GSH), superoxide dismutase (SOD) and horseradish peroxidase (HRP). NADH induced lipid peroxidation system was taken as an in vitro model. The results indicated that GSH could partly inhibit the generation of free radicals at low concentration, while SOD and HRP at proper ration could completely scavenge free radicals, thus prevent mitochondria lipid peroxidation from the damage of lipid peroxidation. |
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| Keywords: | ESR Spin trapping Antioxidant Mitochondria |
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