A high-throughput screening assay for hydroxynitrile lyase activity |
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| Authors: | Andexer Jennifer Guterl Jan-Karl Pohl Martina Eggert Thorsten |
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| Affiliation: | Institute of Molecular Enzyme Technology, Heinrich-Heine University Düsseldorf, Forschungszentrum Jülich, 52426, Jülich, Germany. |
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| Abstract: | A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches. |
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