1.Department of Chemistry,Government College University,Lahore,Pakistan;2.Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information Technology,Lahore,Pakistan;3.Department of Environmental Sciences,COMSATS Institute of Information Technology,Vehari,Pakistan
Abstract:
Ionic liquid coated nanoparticles (IL-NPs) consisting of zero-valent iron are shown to display intrinsic peroxidase-like activity with enhanced potential to catalyze the oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This results in the formation of a blue green colored product that can be detected with bare eyes and quantified by photometry at 652 nm. The IL-NPs were further doped with bismuth to enhance its catalytic properties. The Bi-doped IL-NPs were characterized by FTIR, X-ray diffraction and scanning electron microscopy. A colorimetric assay was worked out for hydrogen peroxide that is simple, sensitive and selective. Response is linear in the 30–300 μM H2O2 concentration range, and the detection limit is 0.15 μM.
Graphical abstract Schematic of ionic liquid coated iron nanoparticles that display intrinsic peroxidase-like activity. They are capable of oxidizing the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This catalytic oxidation generated blue-green color can be measured by colorimetry. Response is linear in the range of 30–300 μM H2O2 concentration, and the detection limit is 0.15 μM.
