| 血红蛋白在Span 80/PEG 400/H2O囊泡体系中的结构性质 |
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| 引用本文： | 王元有,华伟,刘天晴.血红蛋白在Span 80/PEG 400/H2O囊泡体系中的结构性质[J].物理化学学报,2011,27(8):1907-1912. |
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| 作者姓名： | 王元有 华伟 刘天晴 |
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| 作者单位： | 1. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu Province, P. R. China;
2. Department of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, Jiangsu Province, P. R. China |
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| 基金项目： | 国家自然科学基金(20573091)资助项目~~ |
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| 摘 要： | 通过紫外-可见吸收光谱、荧光光谱、动态光散射、圆二色谱、负染-透射电镜(NS-TEM)和冷冻蚀刻-透射电镜(FE-TEM)等实验方法研究了血红蛋白(Hb)与Span 80/PEG 400/H2O囊泡间的相互作用及其结构特性. 结果表明: Hb易于吸附在囊泡表面, 使得囊泡的表观半径稍有增大; Hb的肽链在囊泡表面能够逐渐伸开, 特征荧光峰强度显著增强, 部分氨基酸残基进一步暴露, α-螺旋结构含量减少, β-折叠和β-转角结构含量增加, 无规卷曲结构含量基本不变. 囊泡体系中Hb的稳定性与囊泡的稳定性有关.
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| 关 键 词： | 血红蛋白 结构 性质 非离子囊泡 |
| 收稿时间： | 2011-02-28 |
| 修稿时间： | 2011-06-21 |
Structural Property of Hemoglobin in Span 80/PEG 400/H_2O Niosome System |
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| WANG Yuan-You,HUA Wei,LIU Tian-Qing.Structural Property of Hemoglobin in Span 80/PEG 400/H_2O Niosome System[J].Acta Physico-Chimica Sinica,2011,27(8):1907-1912. |
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| Authors: | WANG Yuan-You HUA Wei LIU Tian-Qing |
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| Institution: | 1. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu Province, P. R. China;
2. Department of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, Jiangsu Province, P. R. China |
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| Abstract: | The structural property of hemoglobin(Hb) was studied in detail by UV-Vis absorption,fluorescence,circular dichroism,negative staining-transmission electron microscopy(NS-TEM),and freeze etching-transmission electron microscopy(FE-TEM) techniques using a Span 80/PEG 400/H2O niosome system.The obtained results show that Hb is adsorbed onto the surface of niosome.The peptide chain of Hb spreads out and the apparent radius of the niosome,the intensities of the UV-Vis absorption peaks,and the fluorescence peaks... |
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| Keywords: | Hemoglobin Structure Property Niosome |
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