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| 碱蓝6B共振光散射法检测DNA | |
| 引用本文： | 龙云飞,陈小明,伍群丽,杨伟军. 碱蓝6B共振光散射法检测DNA[J]. 光谱学与光谱分析, 2003, 23(3): 458-460 |
| 作者姓名： | 龙云飞 陈小明 伍群丽 杨伟军 |
| 作者单位： | 1. 湘潭大学化学学院,湖南,湘潭,411105;湘潭师范学院化学系,湖南,湘潭,411201 2. 湘潭大学化学学院,湖南,湘潭,411105 3. 湘潭师范学院化学系,湖南,湘潭,411201 |
| 基金项目： | 湖南省自然科学基金资助项目 (0 0JJY2 0 84) |
| 摘 要： | 研究了碱蓝 6B与脱氧核糖核酸在酸性条件下共振光散射特征 ,考察了影响因素和最佳反应条件 ,建立了一种测定纳克级DNA的方法。在四硼酸钠 盐酸 (pH =2 90 )缓冲溶液中 ,线性范围为 10～ 10 0 4 μg·L- 1 ,相关系数为 0 9913,检出限为 4 2 μg·L- 1 ,用于合成样品的测定结果令人满意。 |
| 关 键 词： | 共振光散射光谱 脱氧核糖核酸 碱蓝6B |
| 文章编号： | 1000-0593(2003)03-0458-03 |
| 修稿时间： | 2002-09-06 |
Determination of Deoxyribonucleic Acid with Alkali Blue 6B by Resonance Light-scattering Method |
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| LONG Yun-fei ,,CHEN Xiao-ming,WU Qun-li and YANG Wei-jun. Determination of Deoxyribonucleic Acid with Alkali Blue 6B by Resonance Light-scattering Method[J]. Spectroscopy and Spectral Analysis, 2003, 23(3): 458-460 | |
| Authors: | LONG Yun-fei CHEN Xiao-ming WU Qun-li YANG Wei-jun |
| Affiliation: | Institute of Chemistry, Xiangtan University, Department of Chemistry, Xiangtan Normal University, Xiangtan, China. |
| Abstract: | Characteristics of the interaction between alkali blue 6B with deoxyribonucleic acid (DNA) was studied by resonance light-scattering (RLS) method in the pH 2.90 medium. The effects of interference substances and the optimization of procedure were investigated, and a new method based on RLS was established. The method can be used to determine the DNA at nanogram level. The linear range of the DNA is 10-100.4 micrograms.L-1 with a correlation coefficient of 0.9913. The detection limit is 4.2 micrograms-L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory results. |
| Keywords: | Resonance light-scattering spectroscopy DNA Alkali blue 6B |
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