Enhancing endothelial differentiation of human mesenchymal stem cells by culture on a nanofibrous polycaprolactone/(poly-glycerol sebacate)/gelatin scaffold |
| |
Authors: | Shima Majidansari Negin Vahedi Mahmood Rekabgardan Camellia Ganjoury Najmeh Najmoddin Mohammad Tabatabaei Faraz Sigaroodi Paniz Naraghi-Bagherpour Seyed Amir Ali Taheri Mohammad-Mehdi Khani |
| |
Institution: | 1. Department of Tissue Engineering, Science and Research branch, Islamic Azad University, Tehran, Iran;2. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran;6. Cell Engineering and Biomicrofluidics Systems Lab, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran |
| |
Abstract: | Cardiovascular diseases have always been one of the main causes of death worldwide and eventually one of the major medical concerns. Tissue engineering is promising strategies of treating cardiovascular, which can be an effective approach with the design of appropriate scaffold. In this study, to develop engineering basement membrane for endothelial differentiation with good cell attachment, we produced polycaprolactone (PCL)/poly (glycerol sebacate) (PGS)/gelatin nanofibrous scaffold via electrospinning. Attenuated total reflectance-Fourier transform infrared and the proton nuclear magnetic resonance results confirmed the chemical structure of synthesized PGS. Scanning electron microscope images of the electrospun scaffold revealed that the nanofibers are smooth, continues and uniform. Moreover, due to the presence of hydrophilic functional groups in the scaffold, the contact angle is in the appropriate range for cell adhesion especially endothelial cells. The elastic modulus and ultimate tensile stress of electrospun scaffold were calculated 1.32 ± 0.27 MPa and 1.23 ± 0.18 MPa respectively. Quantitative polymerase chain reaction was performed for evaluation of endothelial differentiation of mesenchymal stem cells cultured on standard plate and fibrous scaffold under chemical stimulation with growth factor. Specific endothelial gene expression results postulated that our modified scaffold could support and significantly promote endothelial differentiation of MSCs. |
| |
Keywords: | cardiovascular tissue engineering electrospinning endothelial differentiation poly (glycerol sebacate) |
|
|