Preparation of thiophilic paramagnetic adsorbent for separation of antibodies |
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Authors: | Ming Qin Chen Zhi Yong Lin Hao Qian |
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Affiliation: | College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China |
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Abstract: | The micron-sized mierospheres with superparamagnetic property were synthesized with vinyl acetate and divinylbenzene by microsuspension polymerization. After the complete alcoholysis, these hydroxyl-functionalized microspheres were activated by divinylfone and modified with mercaptoethanol to prepare the thiophilic magnetic adsorbent, which was used to specifically isolate immunoglobulin G (IgG) from human serum. This thiophilic magnetic adsorbent performed an evident salt-dependent adsorption behavior for IgG. Due to their salt-promoted adsorption towards IgG under high salt concentration, the absorbed antibodies could be extracted in low salt concentration with high purity. |
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Keywords: | Superparamagnetic Thiophilic Microsuspension Immunoglobulin G Isolation |
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