| 葛根药材指纹图谱分析方法研究 |
| |
| 引用本文： | 张雷红,沈小钟,张现涛,赵珍东. 葛根药材指纹图谱分析方法研究[J]. 广东微量元素科学, 2010, 17(7): 29-33 |
| |
| 作者姓名： | 张雷红 沈小钟 张现涛 赵珍东 |
| |
| 作者单位： | 1. 广东食品药品职业学院,广东,广州,510520
2. 广东省中药研究所,广东,广州,510520 |
| |
| 摘 要： | 以安徽产葛根药材为分析对象,选择Agilent ZORBAX SB-C18柱(4.6 mm×250 mm,5μm),甲醇-水梯度洗脱,流量为0.9 mL.min-1,检测波长为250 nm,柱温25℃,建立了葛根药材HPLC指纹图谱。结果表明,10批供试品的保留时间在60 min之内,共有18个典型的共有色谱峰。采用HPLC法获得的葛根药材指纹图谱稳定、可靠、重复性好,能较全面地反映葛根药材的内在质量,可用于葛根药材的质量控制。
|
| 关 键 词： | 葛根 指纹图谱 高效液相色谱法 |
Fingerprint Chromatogram Analysis of Radix Puerariae |
| |
| ZHANG Leihong,SHEN Xiaozhong,ZHANG Xiantao,ZHAO Zhendong. Fingerprint Chromatogram Analysis of Radix Puerariae[J]. Trace Elements Science, 2010, 17(7): 29-33 |
| |
| Authors: | ZHANG Leihong SHEN Xiaozhong ZHANG Xiantao ZHAO Zhendong |
| |
| Affiliation: | 1 (1. Guangdong Food and Drug Vacational College, Guangzhou 510520, China; 2. Guangdong Research Institute of Chinese Traditional Medicine, Guangzhou 510520, China) |
| |
| Abstract: | To establish the HPLC - fingerprint chromatogram of Radix Puerariae, Radix Puerariae from Anhui province was used, under the conditions of Agilent ZORBAX SB - C18 column （4. 6 mm × 250 mm, 5 μm） with a solvent system consisting of methanol - water by gradient elution, the flow rate was O. 9 mL · min-1, and the detecting wavelength was 250 nm, the column temperature was 25 ℃, the HPLC fingerprint chromatogram of Radix Puerariae was set up. The results the retention time in 10 batches of samples was less than 60 min and the tlngerpr/nt had 18 typical common peaks. It concludes the fingerprint obtained by HPLC is stable and reliable, and has a good reproducibility. It can be used to control the quality of Radix Puerariae. |
| |
| Keywords: | Pueraria lobata （Willd） Ohwi fingerprint HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
