Increased synthesis of ajmalicine and catharanthine by cell suspension cultures ofCatharanthus roseus in response to fungal culture-filtrates |
| |
| Authors: | DiCosmo F Quesnel A Misawa M Tallevi S G |
| |
| Institution: | (1) Centre for Plant Biotechnology, University of Toronto, M5S 1A1 Toronto, Ontario, Canada;(2) Department of Botany, University of Toronto, M5S 1A1 Toronto, Ontario, Canada;(3) Institute of Biomedical Engineering, University of Toronto, M5S 1A1 Toronto, Ontario, Canada;(4) Allelix Inc., 6850 Goreway Drive, L4V 1P1 Mississauga, Ontario, Canada |
| |
| Abstract: | The ammonium sulfate-precipitated fraction from mycelia and culture-filtrates and the crude, cell-free culture filtrates from
the growth medium of the fungiChrysosporium palmorum, Eurotium rubrum, Micromucor isabellina, andPythium aphanidermatum when aseptically added to cell suspensions ofCantharanthus roseus caused a rapid and dramatic increase in indole alkaloid biosynthesis. Up to 400 μg/L ajmalicine and 600 μg/L catharanthine
were detected in C.roseus cell suspension grown in the presence of theM. isabellina fungal culture filtrate for 3 d. Untreated cells produced only trace levels of ajmalicine and catharanthine per liter of
cell suspension after 15 d of culture. |
| |
| Keywords: | Catharanthus roseus fungal elicitation ajmalicine catharanthine |
| 本文献已被 SpringerLink 等数据库收录！ |
|
