Sugar-assisted ligation of N-linked glycopeptides with broad sequence tolerance at the ligation junction |
| |
Authors: | Brik Ashraf Ficht Simon Yang Yu-Ying Bennett Clay S Wong Chi-Huey |
| |
Institution: | Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. abrik@bgu.ac.il |
| |
Abstract: | A novel method for the synthesis of N-linked glycopeptides using the sugar-assisted ligation strategy from cysteine free peptides is presented. The ligation junction tolerates a variety of amino acids, favoring less hindered amino acids and those with side chains that could serve as a general base in the ligation pathway. Since our approach allows the ligation of difficult junctions, the method could be applied to the synthesis of large peptides by enzymatic removal of the sugar moiety. Alternatively, more complex glycopeptides can be synthesized using glycosyltransferases. Together, this sequence of reactions should be amenable to the synthesis of glycopeptides and glycoproteins and their deglycosylated products. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|