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Scanning photoluminescent spectroscopy of bioconjugated quantum dots |
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| Authors: | G. Chornokur S. Ostapenko E. Oleynik C. Phelan N. Korsunska T. Kryshtab J. Zhang A. Wolcott T. Sellers |
| Affiliation: | 1. Department of Radiology Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain;2. Department of Cardiology, Hospital Sierrallana, Torrelavega, Cantabria, Spain;3. Department of Internal Medicine, Hospital Sierrallana and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Torrelavega, Cantabria, Spain;4. Quality Unit, Hospital Universitario Marques de Valdecilla, Santander, Cantabria, Spain;5. Department of Radiology, Hospital Sierrallana, Torrelavega, Cantabria, Spain;6. Department of Radiology, Hospital Universitari Vall d´Hebron, Barcelona, Catalonia, Spain;7. Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain;8. Department of Cardiology, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain |
| Abstract: | We report on the application of the bio-conjugated quantum dots (QDs) for a “sandwich” enzyme-linked immunosorbent assay (ELISA) cancer testing technique. Quantum dot ELISA detection of the cancer PSA antigen at concentrations as low as 0.01 ng/ml which is ～50 times lower than the classic “sandwich” ELISA was demonstrated. Scanning photoluminescence (PL) spectroscopy was performed on dried ELISA wells and the results compared with the same QD samples dried on a solid substrate. We confirmed a “blue” up to 37 nm PL spectral shift in a case of QDs conjugated to PSA antibodies. Increasing of the “blue” spectral shift was observed at lower PSA antigen concentrations. The results can be used to improve sensitivity of “sandwich” ELISA cancer antigen detection. |
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