7-Triazolylcoumarin-based fluorescent tag system for stepwise,comparative assessment of small molecule microarrays |
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| Authors: | Moon-Kook Jeon Myoung-Ku Kang Koon Ha Park |
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| Institution: | 1. Division of Drug Discovery Research, Korea Research Institute of Chemical Technology, PO Box 107, Sinseongno 19, Yuseong-gu, Daejeon 305-600, Republic of Korea;2. Department of Chemistry, Chungnam National University, Daehakro 99, Yuseong-gu, Daejeon 305-764, Republic of Korea |
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| Abstract: | The potential use of a fluorescent tag system based on 7-(1H-1,2,3-triazol-4-yl)coumarin fluorophore having a fluorous moiety and a polyethylene glycol (PEG) spacer at opposite ends as a tool for a stepwise and comparative evaluation of the fabrication process of small molecule microarrays was illustrated by the qualitative analysis of the results of the fluorescence detection obtained from the microarray experiments using the tagged biotins and streptavidin-Cy3 (and avidin-Cy5) as the binding partners. |
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