Photo-triggered generation of a free thiol group on DNA: application to DNA conjugation |
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| Authors: | Tadao Takada Yuta Kawano Mitsunobu Nakamura Kazushige Yamana |
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| Affiliation: | Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan |
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| Abstract: | DNA oligomers possessing a 2-nitrobenzyl (NB) protected thiol group have been prepared. The photo-remove of the NB to generate a free thiol group in DNA has been analyzed by using reverse-phase HPLC and denaturing gel electrophoresis. The photo-triggered generation of the thiol function in DNA was applicable in the light-initiated ligation of thiol-modified DNA oligomers and Au–DNA conjugation. |
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