铕-速尿-TOPO荧光配合物的研究及速尿的测定

在pH7.8的缓冲溶液中,铕离子、速尿和三辛基氧膦(TOPO)反应生成稳定的三元配合物,该配合物溶解在Triton X-100胶束中。以280 nm光波激发,配合物发射出铕离子的特征荧光。分析了三元配合物的紫外及荧光光谱,对配合物的生成及TOPO和Triton X-100的荧光增敏机理进行了探讨。采用时间分辨荧光法测定配合物的荧光强度,其荧光强度和速尿的浓度在一定范围内呈线性关系,建立了工作曲线的线性回归方程。方法的检出限为6.6×10-8mol/L,测定精度RSD为1.3%(4.00×10-6mol/L,n=11)。用此方法测定尿液中痕量速尿,回收率为93.0%～103%。

Research of europium-furosemide-TOPO fluorescent complex and determination of furosemide

In Tris-HCl buffer solution of pH 7.8 europium ions,furosemide(FR) and trioctylphosphine oxide(TOPO) formed a stable ternary complex which was dissolved in triton X-100 micelles.The complex emits characteristic fluorescence of europium ion with excitation wavelength at 280 nm.According to the UV and fluorescence spectra of the ternary complex the formation of the complex and mechanism of fluorescence enhancement caused by TOPO and Triton X-100 were discussed.Taking the advantage of long fluorescent lifetime...