蛋白质、凝胶电泳及其分析应用

蛋白质是重要的生物高分子，具有催化、传输、运动、防御和调节等重要生理功能，其分离、纯化和表征对理解和利用它们在生命过程中的作用具有重大理论意义和实际价值。凝胶电泳是蛋白质测定中应用最广泛和最强有力的工具。本文讨论凝胶电泳方法及其在蛋白质分析方面的新进展。首先介绍蛋白质的性质和功能，然后讨论蛋白质试样制备方法，最后评述各种凝胶电泳分离技术及其在蛋白质分离、纯化和表征等方面的应用。

Protein,Gel Electrophoresis and the Application of Gel Electrophoresis in Protein Analysis

As very important biopolymers proteins have many biological functions such as caalysis,transportstion,movement,defence and regulation,etc.The isolation,purification and chsracterization of proteins are of great theoretical importance and practical value in understanding and utilizing their specific functions and activities in life process.Gel electrophoresis is the most widely used and the most powerful tool in protein determination.This article discusses gel electrophoresis and its application in protein analysis.First,it introduces propertics and fractions of proteins,then discusses the reparation of protein samples,and finally,reviews the gel electrophoresis techniques and teir application in protein iSolation,purfication and chsracterization.