Liquid fluidized bed starter culture ofTrichoderma reesei for cellulase production |
| |
Authors: | R. P. Tengerdy W. H. Rho A. Mohagheghi |
| |
Affiliation: | 1. Department of Microbiology, Colorado State University, 80523, Fort Collins, CO 2. Biotechnology Branch, Solar Energy Research Institute, 1617 Cole Blud., 80407, Golden, CO
|
| |
Abstract: | A starter culture ofTrichoderma reesei (Rut-C30) prepared in a liquid fluidized bed reactor (LFBR) gave better growth and greater cellulase production in submerged fermentation than a conventional shake flask inoculum. The LFBR starter was prepared by first coatingT. reesei spores to 0.25 mm size corncob (1.0x108g-1) in a medium containing 1.0% corncob, 0.5 gL-1 xylose and 0.1 gL-1 lactose in a balanced salt solution, then fluidizing the particles in the LFBR for 36 h to allow germination of the spores, and covering the particles with an approx 30 μm thick biofilm. This biofilm that developed in constant adherence to the lignocellulosic carrier, apparently became well adapted to grow rapidly on insoluble cellulose substrates (Solca Floc), and had the enzymes of the cellulase complex induced for increased cellulase production. The LFBR starter used in a stirred tank reactor (STR) gave 15 gL-1 biomass production and 6.5 IU mL-1 overall cellulase activity with a volumetric productivity of 64 IU L-1h-1 in a 5 d fermentation, compared with a 7 d shake flask inoculum that gave 11 gL-1 biomass and 3.2 IU mL-1 cellulase activity, with a volumetric productivity of 31IU L-1h-1. The LFBR starter culture retained its viability in dry storage for 6–9 mo. |
| |
Keywords: | KeywordHeading" >Index Entries Cellulase, production liquid fluidized bed reactor (LFBR) Trichoderma reesei, in cellulose fermentation cellulose, as substrate for fermentation corn cob, as carrier in LFBR |
本文献已被 SpringerLink 等数据库收录! |
|