| 一体化微流控芯片的酶固定化技术 |
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| 引用本文： | 田玉平,吴会灵,陈淑桂,杨芃原. 一体化微流控芯片的酶固定化技术[J]. 高等学校化学学报, 2004, 25(5): 847-849 |
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| 作者姓名： | 田玉平 吴会灵 陈淑桂 杨芃原 |
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| 作者单位： | 1. 吉林大学化学学院, 长春 130026;2. 复旦大学化学系, 上海 200433 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 0 2 990 3 0 )，国家“九七三”计划项目 (批准号:0 0 1CB5 10 2 0 2 )，国家“八六三”计划项目 (批准号 :2002BAC11A11)资助 |
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| 摘 要： | 20世纪90年代，Manz等提出的微全分析系统越来越受到关注，聚二甲基硅氧烷[Poly(dimethylsiloxane)，PDMS]材料具有透光性能好和绝缘等优点，并可通过浇铸法制作一体化的芯片，从而有效地解决芯片的封合问题。
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| 关 键 词： | 微流控芯片 固定化酶 聚二甲基硅氧烷 |
| 文章编号： | 0251-0790(2004)05-0847-03 |
| 收稿时间： | 2003-09-18 |
Enzyme Immobilization Technique in Microfluidic Channel on Integrated Chip |
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| TIAN Yu-Ping ,,WU Hui-Ling ,CHEN Shu-Gui ,YANG Peng-Yuan. Enzyme Immobilization Technique in Microfluidic Channel on Integrated Chip[J]. Chemical Research In Chinese Universities, 2004, 25(5): 847-849 |
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| Authors: | TIAN Yu-Ping WU Hui-Ling CHEN Shu-Gui YANG Peng-Yuan |
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| Affiliation: | 1. College of Chemistry, Jilin University, Changchun 130026, China;2. Department of Chemistry, Fudan University, Shanghai 200433, China |
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| Abstract: | A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running. |
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| Keywords: | Microfluidic chip Immobilized enzyme PDMS |
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