Polarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus |
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Authors: | Young Woo Han Abi G Aleyas Junu A George Seon Ju Kim Hye Kyung Kim Hyun A Yoon Dong Jin Yoo Seong Ho Kang Koanhoi Kim and Seong Kug Eo |
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Affiliation: | 1Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.;2Department of Chemistry, Seonam University, Namwon 590-711, Korea.;3Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University, Jeonju 561-756, Korea.;4Department of Pharmacology, School of Medicine, Pusan National University, Busan 602-739, Korea. |
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Abstract: | Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protective immunity against a lethal challenge. Among three constructs, recombinant adenovirus expressing gB (rAd-gB) was found to induce the most potent immunity biased to Th1-type, as determined by the IgG isotype ratio and the profile of the Th1/Th2 cytokine production. Conversely, the gC-expressing adenovirus (rAd-gC) revealed Th2-type immunity and the gD-expressing adenovirus (rAd-gD) induced lower levels of IFN-γ and IL-4 production than other constructs, except IL-2 production. Mucosal delivery of rAd-gB induced mucosal IgA and serum IgG responses and biased toward Th2-type immune responses. However, these effects were not observed in response to systemic delivery of rAd-gB. In addition, rAd-gB appeared to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route. These results suggest that administration of replication-incompetent adenoviruses can induce different types of immunity depending on the expressed antigen and that recombinant adenoviruses expressing gB induced the most potent Th1-biased humoral and cellular immunity and provided effective protection against PrV infection. |
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Keywords: | adenoviruses human herpesvirus 1 Suid Th1 cells Th2 cells vaccination |
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