Molecular beacon probes for the detection of cisplatin-induced DNA damage |
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Authors: | Zahra?J?Shire Email author" target="_blank">Glen?R?LoppnowEmail author |
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Institution: | (1) Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada; |
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Abstract: | Cisplatin (cis-diamminedichloroplatinum(II)) causes crosslinking of DNA at AG and GG sites in cellular DNA, inhibiting replication,
and making it a useful anti-cancer drug. Several techniques have been used previously to detect nucleic acid damage but most
of these tools are labour-intensive, time-consuming, and/or expensive. Here, we describe a sensitive, robust, and quantitative
tool for detecting cisplatin-induced DNA damage by using fluorescent molecular beacon probes (MB). Our results show a decrease
of fluorescence in the presence of cisplatin-induced DNA damage, confirmed by matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry (MALDI-TOF MS). The decrease in fluorescence upon damage scales with the number of AG and
GG sites, indicating the ability of MB to quantitatively detect DNA damage by cisplatin. |
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