3种人工结香方法所得沉香挥发性成分的SPME/GC-MS分析

采用固相微萃取(SPME)富集生物、化学及物理(火钻)法所得沉香的挥发性成分,用气相色谱-质谱联用技术(GC-MS)分离鉴别,然后对这3种人工结香方法进行评价。研究发现沉香的挥发性成分主要由倍半萜、芳香族等化合物组成,但由于结香方法不同,其化学成分及含量有很大差别。以沉香的6种特征性有效成分苄基丙酮、对甲氧基苄基丙酮、沉香呋喃、沉香螺旋醇、苍术醇、白木香醛及其含量作为评价沉香品质的主要指标,其中火钻法所得沉香中沉香螺旋醇的相对含量最高,约为5.5%;生物结香法所得沉香的特征成分的总含量最高,为16.6%;其次为火钻法所得的沉香,约为13.6%;化学法所得沉香的特征成分总含量相对较低。结果表明生物法的结香效果最好,其次为火钻法,均优于化学法。

SPME/GC-MS Analysis on Volatile Constituents of Agarwood Produced by Three Artificial Inducing Methods

The volatile compositions of agarwood were produced by biological,chemical and physical(flame drilled) methods,then enriched by solid phase microextraction and isolated and identified by GC-MS.The quality of the agarwood produced was evaluated.Investigation showed that the volatile compositions of the agarwood were all composed of sesquiterpenoids and aromatic compounds,but the chemical compositions and contents of the agarwood were quite different because of the different artificial inducing methods.The six characteristic components,i.e.agarofuran,hinesol,4-phenyl-2-butanone,p-methoxybenzylacetone,agarospirol and baimuxinal,and their contents were used as the evaluation indexes for the agarwood quality.The content of agarospirol in the flame drilled agarwood was the highest,which was about 5.5%.The total content of characteristic components in the agarwood produced by biological method was the highest,which reached to 16.6%,followed by that of the flame drilled agarwood,which was around 13.6%,and the agarwood produced by chemical method was the lowest.The result showed the biological method has the best agarwood quality,the flame drilled method was slightly poor,and they were both superior to the chemical method.