蛋白质溶胶-凝胶包埋法分子印迹复合膜的制备及渗透机理研究

分别以牛血红蛋白、牛血清白蛋白和溶菌酶3种蛋白质为模板分子, 采用表面涂布的方法制备了在Nylon微孔滤膜表面覆盖有聚丙烯酰胺凝胶层的分子印迹复合膜, 并用扫描电镜对制备的分子印迹膜的表面形态和孔结构进行了表征, 发现支撑膜的表面及内部微孔表面均被一层丙烯酰胺凝胶所覆盖. 对用不同蛋白为模板制备的分子印迹膜进行了这3种蛋白的单一组分和双组分混合溶液渗透实验. 结果表明, 各蛋白底物在印迹膜上的渗透规律是特异性的识别位点和尺寸效应共同作用的结果. 特异性识别位点会选择性地识别模板分子, 从而使其渗透速度减慢; 尺寸效应主要体现在底物蛋白的体积越小其渗透越快.

Preparation of Protein Molecularly Imprinted Composite Membrane by Sol-gel Coating Method and Its Permeation Mechanism

Composite MIMs were prepared by poly[acrylamide] sol-gel coated Nylon microfiltration membranes with BHb,BSA and LYZ as the template molecules,respectively.The surface and cross-section morphology of the MIMs were studied by scanning electron microscopy.It is found that both the outer and inner surface of the Nylon membrane were coated by the sol-gel.The permeation experiments were carried out with both the single template and the double components containing the template and its competitive analogue.The results of permeation experiments show that the permeation of the template is attributed to both the size effect and its complementary functional groups and shape with the imprinted sites.The specific imprinted sites could selectively rebind the template,resulting in the slower permeation of the template.And the size effect means that the smaller the size is,the faster the template transports.