| 荧光偏振免疫分析测定磺胺甲基嘧啶研究 |
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| 引用本文： | 王战辉,张素霞,沈建忠. 荧光偏振免疫分析测定磺胺甲基嘧啶研究[J]. 光谱学与光谱分析, 2008, 28(7): 1621-1625. DOI: 10.3964/j.issn.1000-0593.2008.07.041 |
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| 作者姓名： | 王战辉 张素霞 沈建忠 |
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| 作者单位： | 中国农业大学动物医学院，北京 100094 |
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| 摘 要： | 磺胺甲基嘧啶(sulfamerazine，SMR)的芳伯胺基与荧光素异硫氰酸钠异构体Ⅰ(fluorescein isothiocyanate, FITC)的异硫氰基在常温下可以发生偶联反应，经薄层色谱分离之后，可以得到SMR的FITC荧光标记物(Tracer)SMR-FITC。应用荧光偏振免疫分析(fluorescence polarization immunoassay, FPIA)，以SMR单克隆抗体为竞争免疫试剂，优化了检测条件，建立了SMR的FPIA检测方法。在选定的条件下，SMR的半数抑制量(IC50)为23.4 ng·mL-1，最低检测限量为2.3 ng·mL-1，检测范围为5.4到218.8 ng·mL-1，可以达到食品中SMR的最高残留限量要求。同时对16种磺胺类药物的交叉反应做了初步研究，结果显示对SMR、磺胺二甲基嘧啶(sulfamethazine, SMZ)、磺胺嘧啶(sulfadiazine, SDZ)交叉反应率分别为100%，25%和8.6%，对其他磺胺类药物的交叉反应率均低于5%。
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| 关 键 词： | 荧光偏振免疫分析 磺胺甲基嘧啶 荧光标记物 交叉反应率 |
| 收稿时间： | 2007-01-18 |
Development of a Fluorescence Polarization Immunoassay for Sulfamerazine |
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| WANG Zhan-hui,ZHANG Su-xia,SHEN Jian-zhong. Development of a Fluorescence Polarization Immunoassay for Sulfamerazine[J]. Spectroscopy and Spectral Analysis, 2008, 28(7): 1621-1625. DOI: 10.3964/j.issn.1000-0593.2008.07.041 |
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| Authors: | WANG Zhan-hui ZHANG Su-xia SHEN Jian-zhong |
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| Affiliation: | College of Veterinary Medicine, China Agricultural University, Beijing 100094, China |
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| Abstract: | A fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the determination of sulfamera (SMR) was developed. The fluorescein-labeled SMR and sulfamethazine (SMZ) were synthesized and purified by thin layer chromatography (TLC). The optimized SMR FPIA had a dynamic range from 5.4 to 218.8 ng x mL(-1) with IC50 value of 23.4 ng x mL(-1) and a detection limit of 2.3 ng x mL(-1). The specificity of the FPIA for SMR was investigated using other 16 sulfonamides and the cross-reactivity for SMR, SMZ and sulfadiazine (SDZ) was 100%, 25% and 8.6%, respectively. |
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| Keywords: | Fluorescence polarization immunoassay Sulfamerazine Tracer |
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