分子烙印亲和色谱与质谱联用实现中草药活性成分分离鉴定一体化

以骆驼蓬种籽中抗肿瘤活性化合物哈尔明及哈马灵的结构类似物哈尔满作为模 板，用非共价键法制备了对哈尔明及哈马灵具有强亲和性的分子烙印聚合物。此分 子烙印聚合物作为液相色谱固定相与大气压电离飞行时间质谱联用，直接分离鉴定 了草药骆驼蓬种籽甲醇粗提物中所含的哈尔明及哈灵两种抗肿瘤活性成分。实验结 果证明了通过分子烙印亲和色谱与质谱联用方法，快速有效地对中草药活性成分分 离鉴定是可能是。

Efficient Separation and Identification of Active Compounds from Herb by Combining Molecular Imprinting Affinity Chromatography with Mass Spectrometry

To avoid leaking disturbance of the residual template molecules in the application of the molecular imprinted polymer, target analogue (harman) imprinted polymer was prepared with affinity for harmaline and harmine, which exist in Peganum nigellastrum and show antitumor activity. The anti-tumor components, harmaline and harmine, in the crude extract of Peganum nigellastrum were separated and identified simultaneously from the complex matrix by connecting the molecular imprinting affinity column with a positive atmospheric pressure chemical ionization (API) time-of-flight (TOF) mass spectrometry (MS). This result demonstrated the feasibility of efficient separation and identification of active components by combining molecular imprinting affinity liquid chromatography (LC) with mass spectrometry (MS).