| Abstract: | A new fluorescent probe with a long-wavelength emission and multiple hydrogen bond sites for guanine, 3-(4-chloro-6-p-nitrophenoxy-1,3,5-triazinylamino)-7-dimethylamino-2-methylphenazine (CNTDP), was designed and synthesized by using cyanuric chloride as a molecular scaffold, neutral red as a fluorophore and p-nitrophenol as an assistant unit. The recognition behavior of CNTDP for guanine and its spectroscopic properties in different solvents were investigated. It was found that the probe's fluorescence can be selectively quenched by guanine instead of thymine, indicating that fully complementary hydrogen bonding plays a key role in such a recognition process. In addition, the fluorescence quenching mechanism of the probe by guanine and the electronic effects of neutral red, triazine ring and p-nitrophenol moieties on the fluorescence of the whole molecule were also discussed. |
