Fluorescence Energy Transfer Study of Interstrand DNA Cross-linking Caused by Rigid Bisintercalator |
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| Authors: | Bernard Juskowiak Elzbieta Galezowska Teruhisa Ichihara Shigeori Takenaka Makoto Takagi Kenichi Yoshikawa |
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| Institution: | 1. Department of Analytical Chemistry, Faculty of Chemistry , A. Mickiewicz University , Grunwaldzka 6, Poznan, 60-688, Poland;2. Department of Applied Chemistry, Faculty of Engineering , Kyushu University , Fukuoka, 812-81, Japan;3. Department of Physics, Faculty of Science , Kyoto University , Kyoto, 606-8502, Japan |
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| Abstract: | The intermolecular cross-linking of DNA with a rigid bisintercalator, 1,4-bis(( N -methylquinolinium-4-yl)vinyl)benzene (pMQVB) has been studied using fluorescence resonance energy transfer (FRET), fluorescence anisotropy measurements, and dynamic fluorescence microscopy. Short DNA duplexes, single-labeled with fluorescein (donor) and x-rhodamine (acceptor), were used as energy transfer partners. Due to the quenching effect of pMQVB on the emission of both fluorescein and x-rhodamine, the energy transfer was monitored using the corrected Stern-Volmer plots. The cross-linking ability of pMQVB depended on the ligand structure; the planar E , E isomer cross-linked DNA contrary to the non-planar E , Z isomer. Dynamic fluorescence microscopy observation also demonstrated the ability of pMQVB to cross-link large T4 DNA molecules. |
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| Keywords: | Bisintercalators Cross-linking Dna Fluorescence Energy Transfer |
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