| 限制酶Sma I参与的PCR产物克隆技术 |
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| 引用本文： | 周荣家,郭一清,程汉华,余其兴. 限制酶Sma I参与的PCR产物克隆技术[J]. 武汉大学学报(理学版), 1996, 0(2) |
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| 作者姓名： | 周荣家 郭一清 程汉华 余其兴 |
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| 作者单位： | 武汉大学生命科学学院 |
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| 基金项目： | 中国博士后科学基金,国家自然科学基金,武汉市青年科技晨光计划提供资助 |
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| 摘 要： | 介绍了一种增加连接效率的ＰＣＲ产物克隆技术，即ＳｍａＩ内切酶参与的ＰＣＲ产物与ＳｍａＩ酶切的载体的连接；并通过克隆黄鳝基因组中一段约２５０ｂｐ的ＰＣＲ产物的实例证实了该技术的可靠有效性．
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| 关 键 词： | 黄鳝，PCR扩增，亚克隆 |
An EFFICIENT METHOD FOR CLONING OF PCR PRODUCTS BY Sma I-DIRECTED LIGATION |
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| Zhou Rongjia, Guo Yiqing, Cheng Hanhua, Yu Qixing. An EFFICIENT METHOD FOR CLONING OF PCR PRODUCTS BY Sma I-DIRECTED LIGATION[J]. JOurnal of Wuhan University:Natural Science Edition, 1996, 0(2) |
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| Authors: | Zhou Rongjia Guo Yiqing Cheng Hanhua Yu Qixing |
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| Abstract: | This paper presents data demonstrating an efficient method to increase theyield of recombinants in cloning PCR products. In the example presented, 250 hp PCR product from amplification of MonoPterus albus genomic DNA was blunt-end ligated to a SmaI-cutPUC13 in presence of SmaI. |
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| Keywords: | Monopterus albus PCR amplification subcloning |
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