| Fluorescence imaging mitochondrial copper(Ⅱ) via photocontrollable fluorogenic probe in live cells |
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| 作者姓名： | Liulin Wang Buxiang Chen Pingping Peng Wenbo Hu Zhipeng Liu Xiaohua Pei Weihong Zhao Chengwu Zhang Lin Li Wei Huang |
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| 基金项目： | This work was financially supported by the National Natural Science Foundation of China (Nos. 81672508, 61505076), Natural Science Foundation of Jiangsu Province (No. BK20140951), Key University Science Research Project of Jiangsu Province (No. 16KJA180004) and SICAM Fellowship & Scholarship by Jiangsu National Synergetic Innovation Center for Advanced Materials. |
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| 摘 要： | Monitoring mitochondrial derived copper(Ⅱ) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(Ⅱ). Herein, we have reported the design,synthesis and characterization of photocontrollable fluorogenic probe, M_(Cu)~(-3), which is equipped with a photo-labile group(nitrobenzyl group) and mitochondria targeting unit(triphenylphosphonium salt).This novel probe showed an intense fluorescence enhancement in response to copper(Ⅱ) without interference from other metal cations in the biological condition(p H 6–9). The detection limit is 1.7 ×10~(-7) mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated M_(Cu)~(-3) can visualize mitochondrial copper(Ⅱ) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(Ⅱ) during mitochondria specific detection.
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