Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen |
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Authors: | Yudong Wu Weipan Peng Qian Zhao Jiafang Piao Bo Zhang Xiaoli Wu Hanjie Wang Zhihong Shi Xiaoqun Gong Jin Chang |
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Affiliation: | a School of Life Sciences, School of Materials Science and Engineering, Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology(Tianjin), Tianjin 300072, China;b Department of Neurology, and Tianjin Neurosurgery Institute, Tianjin Huanhu Hospital, Tianjin 300060, China |
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Abstract: | At present, many researchers focused on the point-of-care testing (POCT), a method of disease markers detection without large-scale instruments and specialized persons. However, most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection. Herein, we developed a newly QD-immune fluorescence test strips (QD-IFTS) based on quantum dots (QDs) as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen (CEA), a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis. And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS. Under the optimized reaction conditions, chromatographic time of the newly QD-IFTS was only 25 min, sample volume of the newly QD-IFTS was only 40 μL and the LOD of the newly QD-IFTS was 0.72 ng/mL. In addition, the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples, and the results revealed great potential in clinical POCT of other biomarkers. |
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Keywords: | Point-of-care testing Immunochromatography assay Quantum dots Carcinoembryonic antigen Quantitative detection |
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