Open channel-based microchip electrophoresis interfaced with mass spectrometry via electrostatic spray ionization |
| |
Authors: | Yan Deng Liang Qiao Natalia Gasilova Xin-Xiang Zhang Hubert H. Girault |
| |
Affiliation: | a Beijing National Laboratory for Molecular Sciences(BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China;b Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Switzerland |
| |
Abstract: | The coupling between open channel-based microchip electrophoresis and mass spectrometry via electrostatic spray ionization is proposed for in situ detection of fractionated analytes. Electrophoretic separation is performed in an open channel fabricated in a plastic substrate. The solvent of background electrolyte is evaporated from the open channel because of Joule heating during electrophoresis, leaving the dried electrophoretic bands to be directly analyzed by mass spectrometry via scanning electrostatic spray ionization. Proof-of-concept results are obtained with fluorescent dyes and antibiotics as the test samples, demonstrating an efficient on-chip detection platform based on the electrophoresis and electrostatic spray ionization mass spectrometry. |
| |
Keywords: | Open channel Microchip electrophoresis Interface Electrostatic spray ionization mass spectrometry |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《中国化学快报》浏览原始摘要信息 |
|
点击此处可从《中国化学快报》下载全文 |
|