松毛虫性信息素微量成分鉴定方法的研究

采用气相色谱-质谱分析确认了顺-5-,反-7-十二碳二烯乙酸酯是思茅松毛虫性信息素腺体的主要成分（次要成分由于含量极微且受杂质干扰，未能获得全扫描质谱图）。利用高分辨率毛细管气相色谱分析了思茅松毛虫性信息素腺体提取物的酯基转移和乙酰化反应的衍生物,进一步确认了顺-5-，反-7-十二碳二烯醇是思茅松毛虫性信息素腺体中的微量成分。探讨了功能团相互转换微量化学反应法鉴定松毛虫性信息素腺体中微量成分功能团和立体构型的优点。强调了性信息素微量成分鉴定工作在昆虫化学通讯系统研究中的重要性。

Study on Trace Component in Sex Pheromones of Dendrolimus spp.

Two compounds were isolated, as sex pheromone components, from the abdominal tips of the female pine caterpillar moth, Dendrolimus kikuchii. The major component was (identified) as (Z,E)-5,7-dodecadien-1-yl acetate by gas chromatography and mass spectrometry. There are some difficulties to elucidate the structure of the minor component due to its trace and coelution with other components. The derivatives of alkaline methanolysis and reacetylation of pheromone gland extracts of D. kikuchii were analyzed by high-resolution gas chromatography, which was performed to verify the functional group and stereo isomers of the trace component in the pheromone gland extracts. The trace component was characterized as (Z,E)-5,7-dodecadienol via microchemical reaction. The advantages of the conversion of acetates to corresponding alcohols or of alcohols to the corresponding acetates in identifying the trace component of pheromone gland extracts of D. kikuchii were discussed. The importance of identifying the trace component in pheromone chemical communication system of insects is emphasized.