| 溶解性可调节的酶载体制备和固定化酶的研究 |
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| 引用本文： | 李增吉,马林.溶解性可调节的酶载体制备和固定化酶的研究[J].高等学校化学学报,1991,12(5):695-698. |
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| 作者姓名： | 李增吉 马林 |
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| 作者单位： | 吉林大学化学系，吉林大学化学系，吉林大学酶工程国家开放实验室，吉林大学酶工程国家开放实验室 130023，130023 |
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| 摘 要： | 本文利用自由基沉淀聚合反应,合成了甲基丙烯酸-丙烯酰胺-顺丁烯二酸酐三元共聚物,测定了这些共聚物形成水不溶性的大分子氢键复合物的临界pH值.利用共聚物上的酸酐基团,直接进行了木瓜蛋白酶的固定化,得到了具有液相酶与固相酶两者优点的新型修饰酶。
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| 关 键 词： | 溶解性 酶 载体 固定化酶 |
Preparation of Enzyme Carriers with Regulated Solubility and Studies on Immobilized Enzyme |
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| Li Zeng-ji,Shen Jia-cong.Preparation of Enzyme Carriers with Regulated Solubility and Studies on Immobilized Enzyme[J].Chemical Research In Chinese Universities,1991,12(5):695-698. |
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| Authors: | Li Zeng-ji Shen Jia-cong |
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| Abstract: | The methacrylic acid-acrylamide-maleic anhydride copolymers were prepared by radical precipitate copolymerization in dioxane. The copolymers exhibit the property of reversible precipitation-solubility changes due to their macromolecular complexes formed by hydrogen bonds when the pH values of the solution were changed. The immobilization of papain was carried out by using the copolymer as a carrier without other coupling agents. The immobilized papain shows the insoluble form below pH 3. 8 and soluble form above pH 3. 9. The immobilized papain with the advantages of soluble and in-soluble enzyme was obtained. |
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| Keywords: | Macromolecular complex Regulated solubility Immobilized enzyme |
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