| 铁皮石斛原胚体液体悬浮培养条件 |
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| 引用本文： | 陈军峰,张艳,戴黎鸣,周伟,沈婧菁,开国银,周根余.铁皮石斛原胚体液体悬浮培养条件[J].上海师范大学学报(自然科学版),2007,36(1):69-73. |
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| 作者姓名： | 陈军峰 张艳 戴黎鸣 周伟 沈婧菁 开国银 周根余 |
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| 作者单位： | 上海师范大学,生命与环境科学学院,上海,200234 |
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| 基金项目： | 上海市科委科技计划;上海市教委资助项目;上海理工大学校科研和教改项目 |
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| 摘 要： | 以铁皮石斛种子诱导的原胚体为材料，对液体悬浮培养条件下影响铁皮石斛原胚体增殖和多糖成份累积的因素进行了研究．结果表明，铁皮石斛原胚体在1／2MS＋1．0mg／LNAA，每20mL接种2g，蔗糖浓度3％或含10％香蕉提取物上清液的液体培养基中增殖最佳，可溶性多糖成分累积相对较高．
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| 关 键 词： | 铁皮石斛 原胚体 悬浮培养 增殖 多糖 |
| 文章编号： | 23958184 |
| 修稿时间： | 09 25 2006 12:00AM |
Study on the condition of pro-embryoids suspension culture of Dendrobium candidum |
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| CHEN Jun-feng,ZHANG Yan,DAI Li-ming,ZHOU Wei,SHEN Jing-jing,KAI Guo-yin,ZHOU Gen-yu.Study on the condition of pro-embryoids suspension culture of Dendrobium candidum[J].Journal of Shanghai Normal University(Natural Sciences),2007,36(1):69-73. |
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| Authors: | CHEN Jun-feng ZHANG Yan DAI Li-ming ZHOU Wei SHEN Jing-jing KAI Guo-yin ZHOU Gen-yu |
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| Institution: | College of Life and Environment Sciences,Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | The pro-embryoids ofDendrobium candidum were cultured in liquid suspension medium with different conditions. The propagation of pro - embryoid and polysaccharide accumulation was investigated. The results showed that the 1/2MS medium with 1.0mg/L NAA and 3% sucrose or 10% extract from banana was the most propitious to the proliferation and the accumulation of polysaceharides of the pro-embryoids. Inoculation with 2g pro-embeyoids in 20mL liquid medium was the most suitable density for pro-embryoid proliferation and polysaceharides accumulation. |
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| Keywords: | Dendrobium candidum pro-embryoids suspension culture propagation polysaccharide |
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