Evidence that the (6-4) photolyase mechanism can proceed through an oxetane intermediate |
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| Authors: | Asgatay Saâdia Petermann Christian Harakat Dominique Guillaume Dominique Taylor John-Stephen Clivio Pascale |
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| Institution: | Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 6229, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims Cedex, France. |
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| Abstract: | Using the analogue of TpT methylated at the 3'-end N3 position (Tpm3T), we demonstrate that when the oxetane/(6-4) pathway is precluded, water addition occurs at the 3'-end C6 position of the oxetane intermediate to provide its opening. Photoreversal of this (6-4) photoproduct C6 hydrate brings the first experimental evidence that the (6-4) photolyase repair can proceed through an oxetane intermediate. |
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