Determination of Alachlor in food products by a fluorescence polarization immunoassay |
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| Authors: | Yu. V. Nartova T. N. Ermolaeva M. R. Fleisher R. Abuknesha S. A. Eremin |
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| Affiliation: | (1) Lipetsk Sate Technical University, ul. Moskovskaya 30, Lipetsk, 398600, Russia;(2) Lipetsk Regional Bureau of Forensic Medical Examination, ul. Grishina 17, Lipetsk, 398017, Russia;(3) King’s College London, 150 Stamford Street, London, SE1 9NH, UK;(4) Moscow Sate University, Moscow, 119992, Russia |
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| Abstract: | A procedure for the determination of Alachlor in liquid media using a fluorescence polarization immunoassay (FPIA) was developed. The effects of the structure and purification degree of a tracer (analyte with a fluorescent label) on the analytical signals, sensitivity, and selectivity in the determination of Alachlor were studied. The tracer-antibody binding constants (K T) and antigen-antibody binding constants (affinity constants K Af) were calculated, and optimum immunoreagent pairs for the determination of Alachlor were chosen. The calibration graph was linear over the Alachlor concentration range 0.01–1.0 µg/mL; the detection limit was 8 ng/mL. The procedure was tested in the analysis of model solutions and soy-containing food products (sausages). |
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