| Abstract: | A novel label‐free electrochemical method for protein detection based on redox properties of silver was developed. As recognition elements, thrombin‐binding aptamers were used. Screen printed electrodes modified with silver nanoparticles (AgNP) were employed as a sensing platform for aptasensor devices. The oxidation of silver upon polarization served as a basis for analytical response. Three different thrombin binding aptamers with various surface concentrations were studied. Linear range of aptasensor response corresponded to the 10?9 M to 10?7 M thrombin concentration range and the detection limit was 10?9 M. |
