Homologous and heterologous interactions between catalytic and regulatory subunits of Escherichia coli acetohydroxyacid synthaseⅠand Ⅲ |
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基金项目: | Supported by the National Key Project for Basic Research of China (Grant No. 2010CB126102);;National Natural Science Foundation of China (Grant Nos. 20572053, 20421202 & 20432010);;Ministry of Education of China (Grant No. 104189) |
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摘 要: | Homologous and heterologous interactions between acetohydroxyacid synthase (AHAS) I and Ⅲ from E. coli have been studied by surface plasmon resonance (SPR). The catalytic and regulatory subunits association for AHAS I (KD = 1.13 × 10-7 M) was stronger than that for AHAS Ⅲ (KD = 5.29 × 10-7 M). A strong heterologous association between regulatory and catalytic subunits and heterologous activation of catalytic subunits were observed. SPR results combined with enzyme kinetics indicate that the reconstituted he...
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