| SOME FACTORS AFFECTING [~3H]TYROSINE BINDING TO PLASMA MEMBRANE PREPARATION OF RAT CORPORA LUTEA in vitro |
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| 作者姓名： | 高而威 程治平 |
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| 作者单位： | Department oF Physiology，Harbin Medical University，Department oF Physiology，Harbin Medical University |
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| 基金项目： | This work is supported by the National Scientific Research Fund and National Family Planning Association Fund. |
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| 摘 要： | Temperature, pH, enzymes and ions affected the two types of ~3H]tyrosine binding sites differently. The optimal temperature and pH for the high affinity sites were 24 C and 7.4, and for the low affinity sites 37℃ and 7.0, respectively. The ~3H]tyrosine binding to the high and low affinity sites was not observed at 55℃, nor at pH 6.0 or pH 8.5. Trypsin reduced ~3H]tyrosine binding to the high affinity sites, but had no effect on the binding to the low affinity sites. Neuraminidase reduced ~3H]tyrosine binding to the low affinity sites, but had no effect on the binding to the high affinity sites. None of cycloheximide, kCG, cAMP, and progesterone affected ~3H]tyrosine binding.
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SOME FACTORS AFFECTING [~3H]TYROSINE BINDING TO PLASMA MEMBRANE PREPARATION OF RAT CORPORA LUTEA in vitro |
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| GAO ERWEI AND CHENG ZHIPING.SOME FACTORS AFFECTING [~3H]TYROSINE BINDING TO PLASMA MEMBRANE PREPARATION OF RAT CORPORA LUTEA in vitro[J].Science in China(Chemistry),1988(12). |
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| Authors: | GAO ERWEI AND CHENG ZHIPING |
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| Institution: | Department oF Physiology; Harbin Medical University |
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| Abstract: | |
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| Keywords: | tyrosine receptor binding sites corpora lutea progesterone |
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