脑梗塞急性期胰岛素与血糖水平的研究

选择158例脑梗塞患者为观察组，另选择100例非脑梗塞患者为对照组。24h内抽取两组患者的静脉血测定空腹胰岛素值、空腹血糖值，并计算出胰岛素敏感性指数。对两组的胰岛素值、血糖及胰岛素敏感指数进行统计学比较。结果表明，观察组胰岛素水平、血糖水平明显高于对照组（P＜0．01），胰岛素敏感性指数明显低于对照组（P＜0．01），脑梗塞急性期胰岛素及血糖水平增高，胰岛素敏感性指数降低。     

CC60汽轮机冷态启动汽缸温差大原因分析及处理

仪征化纤热电厂 3、4号双抽机组长期以来存在着冷态启动时上下缸温差过大问题。结合此型机组配汽机构的特点 ,分析了冷态开机时上下缸温差较大的原因 ,从理论上计算出高压油动行程为 6 1mm时 ,能使调节级处上下缸温差控制在合格范围内 ,并在运行中作了验证     

论排球击球过程中的"人-球-网"关系

采用献资料法、观察法等研究方法，论述了排球击球过程中“人-球-网”关系的内涵；阐述了调节“人-球-网”关系的专门化知觉；提出了击球时正确处理好“人-球-网”关系的具体训练措施和方法．     

对置活塞二冲程汽油机分层稀燃组织研究

结合对置活塞二冲程汽油机缸径小、冲程长的特点,提出了进排气侧非对称喷雾方式,并采用AVL-Fire软件对喷雾方向进行优化,实现全负荷工况下的缸内均匀混合;同时研究了部分负荷工况下二次喷射对分层混合气形成和稀燃组织的影响.结果表明,进气侧喷射角增大有利于提高缸内混合气的均匀度,排气侧喷射角增大会导致燃油蒸发率降低;二次喷油比例影响分层混合气浓稀区的分布,二次喷油时刻影响缸内混合气稀区的均匀性.当进气侧喷射角为30°、排气侧喷射角为15°时,选择第一次喷油时刻为220℃ A,第二次喷油时刻为270℃ A且二次喷油比例为20%,可有效组织混合气分层.在部分负荷工况下,采用分层稀混合气相比均匀稀混合气的火焰发展期缩短约30%,快速燃烧期缩短约15%.      

基于电刺激的多模式康复系统设计和研究

针对脑卒中后肢体功能障碍、褥疮及疼痛,分析了临床上常用的治疗参数,设计了多模式电刺激治疗系统。该系统通过触摸屏与主芯片STM32L152RCT6进行通信,控制升压和极性转换电路产生幅值、频率及脉宽均可连续调节的双极性电刺激脉冲,验证了系统的稳定性和镇痛的效果。通过试验验证了系统能稳定工作,且电脉冲幅值与电阻值的变化无关,且能提高受试者的疼痛阈值。能产生临床使用的电刺激疗法的各类参数,有利于临床实验和相关科研的开展。电刺激技术能够缓解人体疼痛,提高疼痛阈值。     

NGF诱导PC12细胞转化为神经样细胞微管相关蛋白2(MAP2)的表达

目的探讨神经生长因子(NGF)诱导PC12细胞分化为神经元样细胞时MAP2的表达变化情况.方法细胞培养6 d后应用共聚焦显微镜观察MAP2表达情况;Western Blot法检测诱导不同时间点MAP2的表达情况.结果免疫组化结果显示:MAP2表达呈阳性;免疫印迹结果显示:MAP2蛋白表达在不同时间点呈动态变化,即随诱导时间延长呈递增趋势.结论 NGF成功诱导PC12细胞分化为神经样细胞,为缺血性脑卒中的体外细胞研究提供细胞参考.     

微量元素与脑卒中(2)

脑卒中是目前中国最重要的公共卫生问题,正以其高发病率、高死亡率对我国造成巨大的社会、经济困难。从脑卒中的流行病学特征、脑卒中发病的危险因素、脑卒中患者的微量元素失衡及微量元素检测的临床意义四个方面阐述了微量元素与脑卒中的关系。     

Contralaterally transplanted human embryonic stem cell-derived neural precursor cells (ENStem-A) migrate and improve brain functions in stroke-damaged rats

The transplantation of neural precursor cells (NPCs) is known to be a promising approach to ameliorating behavioral deficits after stroke in a rodent model of middle cerebral artery occlusion (MCAo). Previous studies have shown that transplanted NPCs migrate toward the infarct region, survive and differentiate into mature neurons to some extent. However, the spatiotemporal dynamics of NPC migration following transplantation into stroke animals have yet to be elucidated. In this study, we investigated the fates of human embryonic stem cell (hESC)-derived NPCs (ENStem-A) for 8 weeks following transplantation into the side contralateral to the infarct region using 7.0T animal magnetic resonance imaging (MRI). T2- and T2*-weighted MRI analyses indicated that the migrating cells were clearly detectable at the infarct boundary zone by 1 week, and the intensity of the MRI signals robustly increased within 4 weeks after transplantation. Afterwards, the signals were slightly increased or unchanged. At 8 weeks, we performed Prussian blue staining and immunohistochemical staining using human-specific markers, and found that high percentages of transplanted cells migrated to the infarct boundary. Most of these cells were CXCR4-positive. We also observed that the migrating cells expressed markers for various stages of neural differentiation, including Nestin, Tuj1, NeuN, TH, DARPP-32 and SV38, indicating that the transplanted cells may partially contribute to the reconstruction of the damaged neural tissues after stroke. Interestingly, we found that the extent of gliosis (glial fibrillary acidic protein-positive cells) and apoptosis (TUNEL-positive cells) were significantly decreased in the cell-transplanted group, suggesting that hESC-NPCs have a positive role in reducing glia scar formation and cell death after stroke. No tumors formed in our study. We also performed various behavioral tests, including rotarod, stepping and modified neurological severity score tests, and found that the transplanted animals exhibited significant improvements in sensorimotor functions during the 8 weeks after transplantation. Taken together, these results strongly suggest that hESC-NPCs have the capacity to migrate to the infarct region, form neural tissues efficiently and contribute to behavioral recovery in a rodent model of ischemic stroke.     

Treatment and nursing values of a new mixed-ligand In(III)-based complex on acute ischemic stroke

In the current research, through employing the dual ligand method, a novel coordination polymer has been produced in success via the reaction between H₂glu and In(NO₃)₃·6H₂O in the existence of the nitrogen-donor chelating 2,2′-bpy ligand in CH₃CN In(III) and DMF mixed solvent, and its chemical formula is In(glu)(Hglu)(2,2′-bpy) (1, 2,2′-bpy is 2,2′-bipyridine and H₂glu is glutaric acid). Its application values on the acute stroke were assessed and the corresponding mechanism was investigated simultaneously. Firstly, the levels of inflammatory response in the astrocytes were evaluated by ELISA by measuring the content of inflammatory cytokines released into cerebrospinal fluid. Additionally, the miRNA199a relative expression levels in astrocytes were measured via exploiting real time RT-PCR. Molecular docking simulation demonstrated that synthesized In ion complex exhibited excellent biological activities, multiple binding interactions were formed by the carboxyl groups on the In ion complex.     

网格笔划密度特征的OPCCR错误自动检测

现有的光学印刷体汉字识别（OPCCR）系统中,汉字识别率虽然已经高达98%以上,但仍然会发生错误识别的情况.通常,这些错误的识别还不能被自动检测,采用人工检测,费时费力,大大降低了实际应用系统的自动化和智能化程度.为此,本论文提出了基于网格笔划密度特征的OPCCR错误的自动检测算法.本算法首先建立标准汉字的网格笔划密度特征的特征库.然后,在OPCCR错误的自动检测时,对光学印刷体汉字图像进行预处理、行分割、列分割得到单个汉字图像,提取单个汉字图像的网格笔划密度特征.再把特征和相应的识别出的汉字的特征库中的特征进行相关匹配.于是,根据特征匹配自动检测OPCCR的错误.