噬菌体展示禽流感病毒抗原变异性基因片段文库的构建

构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先， 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次， 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后， 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选.     

Feedback controlled periodic states for different kinds of photorefractive nonlinear response

We investigate analytically and numerically the influence of the type of the photorefractive nonlinear response on the periodic states (attractors) which occur during feedback controlled 2W-coupling and correspond to almost 100% diffraction efficiency of the dynamic index grating. In addition to the case of the local response typical, for example, for LiNbO₃ crystals we consider the cases of nonlocal (diffusive) response (BaTiO₃, SBN) and resonant response (DC-biased BSO, BTO, and BGO crystals). It is shown that the conditions for the transition to the periodic states and their apparent characteristics are strongly different for the two limiting cases above. Received 16 July 2002 / Received in final form 29 October 2002 Published online 4 March 2003     

耦合映像格子中时空混沌的状态反馈控制

利用状态反馈的方法，实现了耦合映像格子中时空混沌的稳定控制.反馈方式可以是没有延迟的，也可以是有延迟的；控制方式可以是连续的，也可以是脉冲的.数值模拟结果证明了状态反馈方法的有效性. 关键词： 耦合映像格子 时空混沌 状态反馈控制     

6σ管理中需要澄清的几个问题

6σ管理作为组织实施连续质量改进的一种经营策略,已引起企业界和学术界的极大兴趣,并在一些企业中得到很好应用。正确实施6σ,需要对其基本概念、假设条件、相关约束等有一个较为全面的了解和认识。本文探讨了6σ文献和应用过程中需要关注的几个问题,其中包括6σ的基本概念和特点;分布假设;过程均值1.5σ漂移的可行性;过程能力指数;短期波动和长期波动;6σ改进和6σ设计等。     

基于机器视觉的车辆时空参数识别

车辆荷载信息是分析桥梁受力和性能评估的重要依据,其中车辆时空信息是准确识别车辆荷载的关键参数。考虑到传统在桥面埋设传感器的方法影响交通、破坏路面,提出了一种基于机器视觉技术的车辆荷载时空参数识别方法,包括车辆的横向定位、车速、车轴数、轴距的识别。基于正面拍摄视频,采用背景差分法来实现车辆检测,进一步通过车牌识别确定车辆在图像中的位置,然后根据车牌与车道线距离来实现车辆横向定位。基于侧面拍摄视频,采用图像标定方法,结合已知长度的辅助检测线来获取车辆的轴距、轴数、车速等车辆参数。通过试验室模型试验和现场试验验证了提出的方法。结果表明,现场试验中车辆横向定位和车速的最大误差分别为2.42%和2.67%,轴距的平均误差为1.63%,识别结果可以为更多其他车辆参数如轴重和总重的识别提供重要的技术支撑。     

Pattern formation in Rayleigh-Bénard convection: Numerical simulation with a coupled-map-lattice model

The various patterns formed in Rayleigh-Bénard convection under different conditions are numerically simulated with a coupled-map-lattice model. In the case of a lower aspect ratio (L/d=5), the simulations vividly depicted the main features of the transition process from a laminar state to a soft turbulence and then to a hard turbulence. In this case, the cellular structure in a horizontal section is also reproduced. In the case of a larger aspect ratio (L/d=30), the simulations successfully reproduced the spiral-defect chaos and target chaos in the initial stage of the pattern evolution which were recently found in experiments. In this case, for a fluid ofPr=1, it is verified in simulations that both the ideal straight rolls and spiral-defect chaos are stable attractors in the same parameter regime, and that the initial condition is a decisive factor for which one of the two is formed. In the same case, for a fluid ofPr=4, the target patterns are dominant instead of the spirals, the sizes and distributions of the target are also notably dependent on the initial condition.     

半刚性基层施工弯沉检测标准研究

根据重庆地区大量实测资料 ,对影响半刚性基层弯沉变异性的各主要因素进行了分析 ,并根据实际需要 ,对施工期实测弯沉的龄期与养生温度的影响进行了修正 ,得出可用于施工检测的弯沉控制标准     

A local positive feedback of the tropical Pacific ocean-atmosphere system on interdecadal timescales

SINCE THE 1990S, THE CLIMATIC VARIABILITY ON INTERDE- CADAL TIME SCALES BECAME THE FOCUS OF THE INTERNATIONAL CLIMATOLOGY RESEARCH MISSIONS[1―3]. ON TIME SCALES OF A DECADE OR MORE, THE OCEAN CIRCULATION PREDOMINATEDHEAT BALANCE AND HYDROLOGICAL CYCLE, S…     

Variability of surface circulation in the South China Sea from satellite altimeter data

11-year satellite altimeter sea surface height （SSH） anomaly data from January 1993 to December 2003 are used to present the dominant spatial patterns and temporal variations of the South China Sea （SCS） surface circulation through Empirical Orthogonal Function （EOF） analysis. The first three EOF modes show the obvious seasonal variations of SSH in the SCS. EOF mode one is generally characterized by a basin-wide circulation. Mode two describes the double-cell basin scale circulation structure. The two cells were located off west of the Luzon Island and southeast of Vietnam, respectively. EOF mode three presents the mesoscale eddy structure in the western SCS, which develops into a strong cyclonic eddy rapidly from July to September. EOF mode one and mode three are also embedded with interannual signals, indicating that the SCS surface circulation variation is influenced by El Nino events prominently. The strong El Nino of 1997/98 obviously changed the SCS circulation structure. This study also shows that there existed a series of mesoscale eddies in the western SCS, and their temporal variation indicates intra-seasonal and interannual signals.