从知识传授到育人:高职“生活中的化学”教学实践*

深挖高职院校公选课“生活中的化学”的育人元素,精心设计教学内容,运用课堂主题辩论、趣味生活实验的设计与展示、探秘生活中的化学等理论知识传授与课内外实践相结合的教学方式,实现全过程育人,充分展现了公选课在立德树人方面的价值。     

Regularity of optimal sets for some functional involving eigenvalues of an operator in divergence form

In this paper we consider minimizers of the functional$\min \{{\lambda }_1(\mathrm{\Omega })+\cdots +{\lambda }_k(\mathrm{\Omega })+\mathrm{\Lambda }|\mathrm{\Omega }|,:\mathrm{\Omega }\subset D\text{ open}\}$ where $D\subset {\mathbb{R}}^d$ is a bounded open set and where $0<{\lambda }_1(\mathrm{\Omega })\le \cdots \le {\lambda }_k(\mathrm{\Omega })$ are the first k eigenvalues on Ω of an operator in divergence form with Dirichlet boundary condition and with Hölder continuous coefficients. We prove that the optimal sets ${\mathrm{\Omega }}^{⁎}$ have finite perimeter and that their free boundary $\partial {\mathrm{\Omega }}^{⁎}\cap D$ is composed of a regular part, which is locally the graph of a $C^{1,\alpha }$-regular function, and a singular part, which is empty if $d<d^{⁎}$, discrete if $d=d^{⁎}$ and of Hausdorff dimension at most $d-d^{⁎}$ if $d>d^{⁎}$, for some $d^{⁎}\in \{5,6,7\}$.     

A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging

Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein–protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.     

土壤肥料学实验课程的教学改革与实践

为全面培育卓越农林人才，提高教学培养质量，本研究结合土壤肥料学的课程内容、教学特点及实验方法，对存在的问题进行了剖析，并结合实际针对存在问题做出了相应改革，适时调整《土壤肥料学》实验课程内容和形式。通过删减验证性实验内容，增设综合性实验，传统部分教学内容实现网络化等三方面对该课程的实验课部分进行了改革与实践，以期提高学生综合运用所学《土壤肥料学》的相关知识来解决生产实际问题的能力。     

地理国情普查地表覆盖内业解译的质量问题研究

地表覆盖内业解译是地理国情普查的一道重要工序。地理国情地表覆盖内业解译现存图斑平面位置精度不足、分类错误多种多样等普遍问题,问题突出典型。文中针对这些问题图文并茂、清晰明了的进行了说明,并从多方面分析问题产生的原因,提出加强对技术指标和采集原则的学习和理解,加强内业与外业的结合,充分发挥解译样本的作用等建议,以有效提高内业解译的质量。     

大学英语教学中中国传统文化渗透路径探究

文化与语言有着密切的联系，一定的文化背景知识有助于促进语言应用能力的提高。大学英语教学的同时也应该是对文化的传授，英语教学不仅是语言知识的教授，更应该是文化知识的传播。从多模态视角出发，探究中国传统文化导入大学英语教学的必要性及途径，有助于提高当代大学生的英语识读能力及文化传播的能力。     

数学专业师范生教学技能的调查研究--以四川文理学院为例

教学技能是教师最重要的专业素质，是师范生在大学期间需要掌握的基础职业技能。通过调查研究表明，数学专业大学生教学基本功发展不平衡，不同年级学生的教学技能水平存在显著差异。教学实践、课改认知、教学交流、自主练习是影响教学技能的四个主因子。男女生在表达技能、教学设计技能、几何作图技能等方面差异显著，而在教材分析、多媒体使用方面不存在显著性差异。要通过完善教学技能培养机制，夯实师范基础技能培养，改革教学技能培养方式等途径，合力提高师范生的教学技能，增强就业竞争力。     

Computational Design of Novel Allosteric Inhibitors for Plasmodium falciparum DegP

The serine protease, DegP exhibits proteolytic and chaperone activities, essential for cellular protein quality control and normal cell development in eukaryotes. The P. falciparum DegP is essential for the parasite survival and required to combat the oscillating thermal stress conditions during the infection, protein quality checks and protein homeostasis in the extra-cytoplasmic compartments, thereby establishing it as a potential target for drug development against malaria. Previous studies have shown that diisopropyl fluorophosphate (DFP) and the peptide SPMFKGV inhibit E. coli DegP protease activity. To identify novel potential inhibitors specific to PfDegP allosteric and the catalytic binding sites, we performed a high throughput in silico screening using Malaria Box, Pathogen Box, Maybridge library, ChEMBL library and the library of FDA approved compounds. The screening helped identify five best binders that showed high affinity to PfDegP allosteric (T0873, T2823, T2801, {"type":"entrez-protein","attrs":{"text":"RJC02337","term_id":"1480409465","term_text":"RJC02337"}}RJC02337, CD00811) and the catalytic binding site (T0078L, T1524, T2328, BTB11534 and 552691). Further, molecular dynamics simulation analysis revealed {"type":"entrez-protein","attrs":{"text":"RJC02337","term_id":"1480409465","term_text":"RJC02337"}}RJC02337, BTB11534 as the best hits forming a stable complex. WaterMap and electrostatic complementarity were used to evaluate the novel bio-isosteric chemotypes of {"type":"entrez-protein","attrs":{"text":"RJC02337","term_id":"1480409465","term_text":"RJC02337"}}RJC02337, that led to the identification of 231 chemotypes that exhibited better binding affinity. Further analysis of the top 5 chemotypes, based on better binding affinity, revealed that the addition of electron donors like nitrogen and sulphur to the side chains of butanoate group are more favoured than the backbone of butanoate group. In a nutshell, the present study helps identify novel, potent and Plasmodium specific inhibitors, using high throughput in silico screening and bio-isosteric replacement, which may be experimentally validated.     

唐代试律诗诗题用典与唐代祥瑞尚奇文化

唐代试律诗诗题对有关祥瑞及灵异之类典故的大量选用,不但体现了唐代统治者借祥瑞文化维护自身统治的治国策略,也表明了尚奇之风在唐代的盛行。