Effects of swimming associated with risedronate in osteopenic bones: An experimental study with ovariectomized rats

Physical activity and risedronate sodium have effects on metabolic bone diseases, maintaining the integrity of bone tissue. Our objective was to evaluate the effects of swimming associated with risedronate as a prophylactic means in osteopenic bone of ovariectomized rats. A total of 24 animals of the Wistar strain were used and separated into four groups containing six animals: Ovariectomy (OVX), ovariectomy and swimming (OVXS), ovariectomy and risedronate (OVXM), ovariectomy, risedronate and swimming (OVXMS). The effectiveness of the treatments were evaluated using the tibia by means of biomechanical, radiographic, histomorphometric analyzes. Statistical analysis was performed by the non-parametric Kruskal–Wallis test (p < 0.05). The OVXM and OVXMS groups showed higher values ​​compared to OVX in maximum strength and rigidity. Microscopic analysis showed increased trabecular bone in the OVXM group in relation to the others, and in the OVXMS compared to OVXS. Proximal densitometry in the OVXM and OVXMS groups showed higher values than the OVX and OVXS groups. There were no significant differences in overall densitometry. In conclusion, when comparing the prophylactic means, risedronate was able to preserve bone mass significantly, unlike exercise where an improvement of bone tissue was observed, although not significant, and when swimming and risedronate are combined the result was even better.     

骨髓干细胞的可塑性及其在栓塞性疾病中的应用

栓塞性血管阻塞在急性心肌梗死、脑梗塞、血栓闭塞性脉管炎、糖尿病足等发病过程中起着重要作用。目前临床上针对这些疾病的治疗手段主要有：药物溶栓、血管旁路移植和介入手术等,多年来其治疗方法无实质性进展。上个世纪九十年代人们发现了干细胞新的生物学特性—可塑性,对干细胞可塑性的研究以及相关技术的发展为心脏病、老年痴呆、帕金森等的治疗带来了希望,也为栓塞性疾病的治疗开辟了新途径,有望成为治疗手段之一。     

复合蛋白锌在儿科疾患方面的应用

儿童缺锌是较普遍的社会现象，济南泉城微量元素生命研究所研制的复合蛋白锌系采用生物技术转化而得到的一种蛋白复合物，属生物合成的有机微量元素，经急性和长期毒性试验均未发现毒副作用，经检测不含兴奋剂，用于临床后，对纠正偏食厌食，反复呼吸道感染和智力低下等近千例儿童疾患，显示了可喜的临床效果，证明是优质有机补锌剂。     

The role of TLC in the screening of inherited metabolic diseases

Summary Thin-layer chromatography (TLC) is a rapid, reliable and inexpensive screening technique for diagnosis of inherited metabolic diseases (IMD). Our screening program encompasses five main situations where the use of TLC is considered to be vindicated: (i) analysis of amino acids; (ii) screening for sugar defects; (iii) detection of pathological oligosaccharidurias; (iv) screening for organic acid disorders; and (v) detection of abnormalities in tryptophan metabolism. Examples are presented of chromatograms obtained from pathological samples. Presented at the 21^st ISC held in Stuttgart, Germany, 15^th–20^th September, 1996     

微量元素与防病治病

介绍了微量元素在防治疾病中的作用及对健康长寿的意义，涉及人体必需微量元素，有害元素，有机锗，微量元素是健康长寿的重要手段以及微量元素与现代文明病。     

蒙药治疗呼吸系统疾病研究进展

回顾了古代“丝绸之路”推动蒙医药的发展历程,分析了现代“一带一路”倡议发展思想下,蒙医药的发展机遇。介绍了蒙医药理论体系中经典的三要素理论,列举了蒙医治疗不同呼吸疾病（慢性阻塞性肺疾病、支气管哮喘、肺癌、肺部感染性疾病、新冠肺炎、肺纤维化等）的主要药物与疗法。从临床应用、有效成分挖掘与作用机制研究等方面,综述了近年来蒙医药在呼吸疾病领域中的应用情况和研究进展。     

金属蛋白研究中几个值得注意的动向

本文叙述了金属蛋白和金属酶研究中近年来几个值得注意的发展动向，即与金属离子相关的疾病（特别是神经退行性疾病）、金属离子在蛋白质的折叠、聚集和装配中的作用，金属伴侣分子、金属蛋白的设计和构建、金属蛋白与DNA相互作用。     

Novel functional activities of anti-dna autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases

DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia.     

Catalytic and DNA-Hydrolyzing Activities of Purified Immunoglobulins IgG from Patients Sera with Autoimmune Disease by Pseudobiospecific Chromatography Using Histidyl-Aminohexyl-Sepharose

Catalytic autoimmune antibodies from patients with antiphospholipid (aPL) antibodies were purified using histidyl-aminohexyl-sepharose gel. The sera were loaded on the columns equilibrated with 25 mM MOPS buffer pH 7.4 and the absorbed proteins were eluted by adding 0.2 M NaCl in the equilibrating buffer. Antibodies purity was evaluated by SDS-PAGE. The purified immunoglobulins G from patients with (aPL) sera by histidyl-aminohexyl-sepharose show DNA-degrading activity of the plasmid pUC19 DNA and catalytic activity in hydrolyzing the peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin.     

阿米西达水悬浮剂防治苹果病害的应用研究

对具有保护和治疗双重功效的仿生性杀菌荆25％阿米西达（Amistar）水悬浮剂在苹果病害管理体系中的应用技术和定位进行了探讨．结果表明，25％Amistar SC除对嘎啦品种有药害外，对红富士、新红星、北斗、澳州青苹等6个骨干品种均无任何不良影响，使用安全；同时。25％阿米西达sc对苹果白粉病、黑星病及斑点落叶病等3种主要病害均具有显著防治效果。使用浓度1：2000～1：2500，防治效果为80％～90％；在广谱性和防效上显著优于目前生产上推广的常规性的苯并咪唑类、三唑类及代森类的杀菌剂品种．应用上重点应在发病前或发病初期施药。间隔7～10d喷1次，连喷3～4，次为宜，同时与其它作用机制的杀菌剂如苯并咪唑类、三唑类等内吸性杀菌剂混用或交替用药．在以红富士、新红星等品种为主栽品种果区的无公害苹果生产病害管理体系中具有极高的推广价值．