Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

A sensitive and reliable LC–MS/MS method was developed and validated for simultaneous quantification of the major components of Huangqi–Honghua extact in rat plasma, including hydroxysafflor yellow A (HSYA), astragaloside IV (ASIV), calycosin‐7‐O‐β‐d ‐glucoside (CAG), calycosin, calycosin‐3′‐O‐glucuronide (C‐3′‐G) and calycosin‐3′‐O‐sulfate (C‐3′‐S). After extraction by protein precipitation with acetonitrile and methanol from plasma, the analytes were separated on a Hypersil BDS C₁₈ column by gradient elution with acetonitrile and 5 mM ammonium acetate. The detection was carried out on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source switched between negative and positive modes. HSYA was monitored in negative ionization mode from 0 to 4.9 min, and ASIV, CAG, calycosin, C‐3′‐G and C‐3′‐S were determined in positive ionization mode from 4.9 to 10 min. The lower limits of quantification of the analytes were 6.25 ng/mL for HSYA, 0.781 ng/mL for CAG and 1.56 ng/mL for ASIV and calycosin. The intra‐ and inter‐assay precision (RSD) values were within 13.43%, and accuracy (RE) ranged from ?8.75 to 9.92%. The validated method was then applied to the pharmacokinetic study of HSYA, ASIV, CAG, calycosin, C‐3′‐G and C‐3′‐S in rat after an oral administration of Huangqi–Honghua extract.     

天花粉蛋白的化学——Ⅴ.粉末状结晶天花粉蛋白的拉曼光谱

本文报道粉末状结晶天花粉蛋白的拉曼光谱。酰胺Ⅰ和酰胺Ⅲ的振动谱带在1680和1250 cm~(-1)。骨架C_α—C—N的振动谱带在965 cm~(-1)。苯丙氨酸残基的特征谱带在1010和1620 cm~(-1)。酪氨酸残基的特征谱带在845和862 cm~(-1)。色氨酸残基的特征谱带在765 cm~(-1)。酰胺Ⅰ和Ⅲ的谱带中,B折叠和无序结构的特征较为明显。表征无序结构的965 cm~(-1)谱带很弱。     

反相高效液相色谱检测丹参药材中4种丹参酮的含量

建立了测定丹参药材中4种丹参酮含量的反相高效液相色谱法，色谱条件：流动相为水(含0．5％三乙胺)-甲醇-四氢呋喃(45／55／5，V／V／V)，流速为1mL/min；PDA检测波长254m；4种成分丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮和二氢丹参酮的加样回收率在95．1％-101．2％之间，线性范围为0．08-2μg。该方法准确，稳定，重现性好。根据该色谱条件，测定了不同产地的丹参药材，结果表明：该色谱方法准确检测了生药中4种丹参酮的含量，适合于丹参药材的质量控制。     

基于FPGA的高速FFT处理器的设计与实现

针对高速实时信号处理的要求,提出了4096点快速傅立叶变换（FFT）处理器在现场可编程门阵列（FPGA）中的设计与实现方法。该方法采用了按频率抽取（DIF）基4算法和6级流水线结构,每级均采用FIFO存储器实现延迟功能,和四路转接器一起共同完成序列的码位抽取。为了避免数据溢出,采用块浮点结构来表示数据,节省了器件资源。实验结果表明,该方法在保证运算精度和实现复杂度的同时,提高了处理器的数据时钟频率和处理速度。     

葛根中葛根素的分离纯化技术进展

葛根素是葛根药材质量控制的主要指标。文章综述了近年来国内葛根提取物中葛根素的分离纯化技术进展。同时,指出β-环糊精键合固定相技术因具有绿色环保和低成本的优点,而成为发展的重点和方向。     

生甘草与大叶海藻配伍后部份药理实验研究

本文观察了生甘草、大叶海藻及其合剂对小鼠的急性毒性作用及镇痛作用,对离体蛙心,家兔离体小肠运动的影响,为甘草与大叶海藻的伍用提供了部份实验数据。     

应用密闭微波辅助提取技术提取玄参中玄参苷

应用密闭微波辅助提取法提取玄参中有效成分玄参苷，考察了微波提取条件（包括溶剂、样品粒径、提取时间、固-液质量体积比和提取次数等）对玄参中提取玄参苷收率的影响，结果表明：溶剂为70％乙醇，玄参的粒径为180目，提取时间为10min，料液比为1：20及提取次数为2次时，玄参苷的提取率最佳。并且与常压微波辅助提取法、超声波提取法及索氏提取法进行了比较，本法具有快速、高效、绿色环保的优点。     

A Survey on Topological Properties of Tiles Related to Number Systems

In the present paper we give an overview of topological properties of self-affine tiles. After reviewing some basic results on self-affine tiles and their boundary we give criteria for their local connectivity and connectivity. Furthermore, we study the connectivity of the interior of a family of tiles associated to quadratic number systems and give results on their fundamental group. If a self-affine tile tessellates the space the structure of the set of its ‘neighbors’ is discussed.     

RP-HPLC同时测定人参总皂苷粗提物中人参皂苷Re和Rh2

利用反相-高效液相色谱法(RP-HPLC)建立了快速测定人参皂苷Re和Rh2含量方法。色谱柱为Agilent Extend C18（4.6×150 mm,5μm）,流动相为乙腈-0.05%乙酸梯度洗脱溶液,流速1.00 mL/min,波长203 nm,柱温30.0℃。人参皂苷Re和Rh2的线性范围分别为0.17~456.6 mg/L和6.00~449.4 mg/L;人参皂苷Re和人参皂苷Rh2的平均加标回收率分别为99.60%和99.78%。本方法快速、灵敏、准确性好,可用于测定人参和西洋参提取物中人参皂苷Re和人参皂苷Rh2的含量。     

赤芍总苷对大鼠血小板功能的影响

为了研究赤芍总苷对血小板聚集功能的影响,采用比浊法观察赤芍总苷对大鼠血小板聚集功能的作用,用放射免疫法观察对大鼠TXB2/6-keto-PGF1α、ET水平的作用,并用硝酸还原酶法测定大鼠血清中NO的浓度。结果发现,赤芍总苷能降低ADP诱导的血小板最大聚集强度,降低大鼠血浆中TXB2的浓度,同时升高血浆中6-keto-PGF1α的水平,调节大鼠血清NO/ET的平衡。实验证明,赤芍总苷有明显降低大鼠血小板聚集的作用。