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1.
TMSOTf-catalyzed intramolecular condensation for catechin and epicatechin units are described. A potential electrophile and a nucleophile were connected with diester linkers and TMSOTf-catalyzed condensation was examined. In comparison with intermolecular catechin and catechin condensation, the intramolecular condensation required high reaction temperature and reversed 3,4-cis product was obtained. The condensed product was transformed into the natural 3,4-cis (+)-catechin-(4β→8)-(+)-catechin dimer. 相似文献
2.
本文通过酶活性测定,荧光光谱和紫外光谱研究了外加Cu2+与烟草多酚氧化酶(简称PPO)的相互作用。结果表明,微量铜的加入能增加酶的活性,[Cu2+]/[PPO]为0.20左右时酶活性最大,[Cu2+]/[PPO]为0.91时,Cu2+开始表现出对PPO活性的抑制;Cu2+对PPO内源荧光的猝灭机制属于形成络合物所引起的静态猝灭,猝灭常数Ksv为8.0375×103L·mol-1;Cu2+的加入使PPO蛋白质分子构象发生变化,α-螺旋含量增加,多肽链及Trp和Tyr残基的芳杂环进一步向分子内收缩,疏水基团之间的疏水作用增强。 相似文献
3.
Leitner Christian Hess Johann Galhaup Christiane Ludwig Roland Nidetzky Bernd Kulbe Klaus D. Haltrich Dietmar 《Applied biochemistry and biotechnology》2002,98(1-9):497-507
The wood-degrading fungus Trametes multicolor secretes several laccase isoforms when grown on a simple medium containing copper in the millimolar range for stimulating
laccase synthesis. The main isoenzyme laccase II was purified to apparent homogeneity from the culture supernatant by using
anion-exchange chromatography and gel filtration. Laccase II is a monomeric glycoprotein with a molecular mass of 63 kDa as
determined by sodium dodecylsulfate polyacrylamide gel electrophoresis, contains 18% glycosylation, and has a pI of 3.0. It oxidizes a variety of phenolic substrates as well as ferrocyanide and iodide. The pH optimum depends on the substrate
employed and shows a bell-shaped pH activity profile with an optimum of 4.0 to 5.0 for the phenolic substrates, while the
nonphenolic substrates ferrocyanide and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) show a monotonic pH profile with
a rate decreasing with increasing pH. 相似文献
4.
烟草中多酚氧化酶的生理生化特征及其活性控制的研究 总被引:29,自引:0,他引:29
对烟草叶中的多酚氧化酶(PPO)进行了部分纯化,对比了叶中PPO在苗期和田间的活性差异,研究了其活性变化规律,发现生长旺盛期PPO活性高于其他时期,研究了烟叶在不同水势条件下PPO活性变化规律,发现水势过高、过低,PPO活性均较低,在一872.78kPa水势条件下,PPO活性最高,所以细胞中的含水量是影响PPO活性的因素之一.研究了PPO在35℃、40℃、45℃、50℃、55℃、60℃、65℃、70℃和75℃等烘烤温度条件下的热敏感性,发现:酶的最适反应温度为35℃;温度由35℃升至75℃,活性逐渐下降,在每一温度下保持5min,活性趋于稳定,用回归法得出了烟叶中PPO的下降百分数与温度之间的关系式.用双倒数作图法比较了NaDiCa、EDTA、巯基乙醇和硫脲4种常用抑制剂对烟叶中PPO活性的抑制强度,结果表明:同一浓度下它们对PPO的抑制强度依次递减,而NaDiCa抑制效率明显高于其他3种抑制剂;CuCl2可以促进PPO活性,用回归法得出了活力增加百分数与二价铜离子浓度的关系式。 相似文献
5.
邻苯三酚自氧化法适于测定SOD活性吗? 总被引:2,自引:1,他引:2
用邻苯三酚自氧化法测定了Cu-ZnSOD(Superoxide dismutase,SOD)及根据天然超氧化物歧化酶活性部位结构设计、合成的一些模型化合物(the models of SOD,MSOD)活性。测试结果表明:Cu-ZnSOD和含Cu(Ⅱ)、Fe(Ⅲ)、Mn(Ⅱ、Ⅲ的SOD模型化合物随浓度升高,有的对邻苯三酚氧化表现为负抑制(即促进邻苯三酚氧化);另一部分化合物在较低浓度表现为抑制,较高浓度表现为负抑制的交替变化;或抑制率的无规则变化。本文对出现负抑制的内在根源进行了探讨,鉴于邻苯三酚自氧化的复杂反应机理;含Cu(Ⅱ)、Fe(Ⅲ)、Mn(Ⅱ、Ⅲ)SOD模型化合物的多酚氧化酶活性和Fenton反应催化活性,及MSOD中金属离子的Lewis酸性,不宜采用邻苯三酚自氧化法测定SOD活性。 相似文献
6.
The polyphenolic profiles and antioxidant effects of Agastache rugosa Kuntze (Banga) flower,leaf, stem and root 下载免费PDF全文
Yun‐Hi Kim Won Sup Lee Soo Jung Lee Jong Sung Jin A. M. Abd El‐Aty Ho‐Chul Shin Jae‐Han Shim Sung Chul Shin 《Biomedical chromatography : BMC》2016,30(2):225-231
Agastache rugosa Kuntze (Korean mint) is used as a spice and in folk medicine in East Asia. The present study identified a total of 18 polyphenols from the flower, leaf, stem and roots of this plant using high‐performance liquid chromatography–tandem mass spectrometry. Fourteen of these compounds had not previously been identified in these plant tissues. Each polyphenol was validated in comparison with external calibration curves constructed using structurally related compounds, with determination coefficients >0.9993. The limits of detection and quantification were 0.092–0.650 and 0.307–2.167 mg/L, respectively. Recoveries of 61.92–116.44% were observed at two spiking levels, with 0.91–11% precision, expressed as relative standard deviation (except anthraquinone spiked at 10 mg/L). Hydroxycinnamic acid was the most abundant compound in the root, while the flowers showed the highest total flavonoid level. Antioxidant activities, determined in terms of reducing power, Fe2+ chelating activity and the radical scavenging activities using α,α‐diphenyl‐β‐picrylhydrazyl and 2‐2?‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid, increased in a concentration‐dependent manner; the highest activity was identified in the stems, followed by leaves > flowers > roots. These findings indicate that A. rugosa is a good source of bioactive compounds and can be used as a functional food. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
7.
《普通高中化学课程标准(2017年版)》强调STSE教育理念。通过对苹果中铁元素、氧气、多酚氧化酶活性对苹果褐变现象影响的实验探究,为提高苹果的使用价值以及食品保鲜技术提供了理论基础,让学习者在"苹果"中感受化学知识的生活化及科学知识的价值。 相似文献
8.
Polyphenol microspheres were prepared by template polymerization of phenol in the presence of horseradish peroxidase as bio‐enzyme catalyst and aqueous 1,4‐dioxane as solvent. The morphology of polyphenol microspheres thus obtained was observed with a scanning electron microscope. Further, polyphenol microspheres as obtained were loaded with palladium to generate polyphenol microsphere‐supported Pd complex. Resultant Pd complex catalyst supported by polyphenol microspheres was characterized by means of Fourier transformation infrared spectrometry, X‐ray diffraction, X‐ray photoelectron spectroscopy and scanning electron microscopy, and its thermal stability was examined. Moreover, the catalytic activity of polyphenol microsphere‐supported Pd catalyst as synthesized for the Heck reactions of acrylic acid with aryl iodides was evaluated. Results indicate that the polyphenol microsphere as obtained has a diameter of about 500 nm. Polyphenol microsphere‐supported Pd catalyst, as synthesized, at a dosage of 0.37 mol% Pd, possesses good catalytic activity for the Heck reactions of acrylic acid with aryl iodides in air at a low temperature of 50°C, and it also exhibits catalytic activity for bromide and activated chlorobenzene. The polyphenol microsphere‐supported Pd complex has good thermal stability, and it can be readily separated and reused; the yield of the reaction of iodobenzene with acrylic acid remains at 82% after five recycle runs, showing promising potential as a catalyst for Heck reactions. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
9.
Thi Thanh Hanh Nguyen Jong-Hyun Jung Min-Kyu Kim Sangyong Lim Jae-Myoung Choi Byoungsang Chung Do-Won Kim Doman Kim 《Molecules (Basel, Switzerland)》2021,26(7)
The main protease (Mpro) is a major protease having an important role in viral replication of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the novel coronavirus that caused the pandemic of 2020. Here, active Mpro was obtained as a 34.5 kDa protein by overexpression in E. coli BL21 (DE3). The optimal pH and temperature of Mpro were 7.5 and 37 °C, respectively. Mpro displayed a Km value of 16 μM with Dabcyl-KTSAVLQ↓SGFRKME-Edans. Black garlic extract and 49 polyphenols were studied for their inhibitory effects on purified Mpro. The IC50 values were 137 μg/mL for black garlic extract and 9–197 μM for 15 polyphenols. The mixtures of tannic acid with puerarin, daidzein, and/or myricetin enhanced the inhibitory effects on Mpro. The structure–activity relationship of these polyphenols revealed that the hydroxyl group in C3′, C4′, C5′ in the B-ring, C3 in the C-ring, C7 in A-ring, the double bond between C2 and C3 in the C-ring, and glycosylation at C8 in the A-ring contributed to inhibitory effects of flavonoids on Mpro. 相似文献
10.
Larisa Ryskalin Stefano Puglisi-Allegra Gloria Lazzeri Francesca Biagioni Carla L. Busceti Linda Balestrini Andrea Fornasiero Stefano Leone Elena Pompili Michela Ferrucci Francesco Fornai 《Molecules (Basel, Switzerland)》2021,26(9)
Curcumin (CUR), a natural polyphenol extracted from rhizome of the Curcuma longa L, has received great attention for its multiple potential health benefits as well as disease prevention. For instance, CUR protects against toxic agents acting on the human body, including the nervous system. In detail, CUR possesses, among others, strong effects as an autophagy activator. The present study indicates that CUR counteracts methamphetamine (METH) toxicity. Such a drug of abuse is toxic by disturbing the autophagy machinery. We profited from an unbiased, low variable cell context by using rat pheochromocytoma PC12 cell line. In such a system, a strong protection was exerted by CUR against METH toxicity. This was associated with increased autophagy flux, merging of autophagosomes with lysosomes and replenishment of autophagy vacuoles with LC3, which instead is moved out from the vacuoles by METH. This is expected to enable the autophagy machinery. In fact, while in METH-treated cells the autophagy substrates α-synuclein accumulates in the cytosol, CUR speeds up α-synuclein clearance. Under the effects of CUR LC3 penetrate in autophagy vacuoles to commit them to cell clearance and promotes the autophagy flux. The present data provide evidence that CUR counteracts the neurotoxic effects induced by METH by promoting autophagy. 相似文献