Development and validation of LC–MS/MS method for amlexanox in rat plasma and its application in preclinical pharmacokinetics

Amlexanox, an anti-inflammatory and anti-allergic agent, has been widely used clinically for the treatment of canker sores, asthma, and allergic rhinitis. Recently, amlexanox has received considerable attention in curing nonalcoholic fatty liver diseases and hepatitis virus infection. Herein, we first established a sensitive high-performance liquid chromatography-tandem mass spectrum (LC–MS/MS) method for the determination of amlexanox in rat plasma. Propranolol was used as the internal standard (IS). Using a simple protein precipitation method, the amlexanox and IS were separated with Capcell Pak C18 column (2.0 × 50 mm, 5 μm) and eluted with water and acetonitrile each containing 0.1% formic acid using gradient elution condition at a flow rate of 0.4 mL·min⁻¹. Amlexanox and IS were detected by a triple quadrupole mass in multiple reactive monitoring (MRM) under the transitions of m/z 299.2 → 281.2 and m/z 259.9 → 116.1 with positive electrospray ionization, respectively. The calibration curves of amlexanox were established with the range of 50 to 2000 ng·mL⁻¹ (r² > 0.99). The validation method consisted of selectivity, accuracy, precision, carryover effect, matrix effect, recovery, dilution effect, and stability. The fully validated method was successfully applied to the pharmacokinetic study of amlexanox in Wistar rats.     

Global existence and time decay of the non-cutoff Boltzmann equation with hard potential

This paper is concerned with the Cauchy problem on the Boltzmann equation without angular cutoff assumption for hard potential in the whole space. When the initial data is a small perturbation of a global Maxwellian, the global existence of solution to this problem is proved in unweighted Sobolev spaces $H^N\left({\mathbb{R}}_{x,v}^6\right)$ with $N\ge 2$. But if we want to obtain the optimal temporal decay estimates, we need to add the velocity weight function, in this case the global existence and the optimal temporal decay estimate of the Boltzmann equation are all established. Meanwhile, we further gain a more accurate energy estimate, which can guarantee the validity of the assumption in Chen et al. (0000).     

Regularity of optimal sets for some functional involving eigenvalues of an operator in divergence form

In this paper we consider minimizers of the functional$\min \{{\lambda }_1(\mathrm{\Omega })+\cdots +{\lambda }_k(\mathrm{\Omega })+\mathrm{\Lambda }|\mathrm{\Omega }|,:\mathrm{\Omega }\subset D\text{ open}\}$ where $D\subset {\mathbb{R}}^d$ is a bounded open set and where $0<{\lambda }_1(\mathrm{\Omega })\le \cdots \le {\lambda }_k(\mathrm{\Omega })$ are the first k eigenvalues on Ω of an operator in divergence form with Dirichlet boundary condition and with Hölder continuous coefficients. We prove that the optimal sets ${\mathrm{\Omega }}^{⁎}$ have finite perimeter and that their free boundary $\partial {\mathrm{\Omega }}^{⁎}\cap D$ is composed of a regular part, which is locally the graph of a $C^{1,\alpha }$-regular function, and a singular part, which is empty if $d<d^{⁎}$, discrete if $d=d^{⁎}$ and of Hausdorff dimension at most $d-d^{⁎}$ if $d>d^{⁎}$, for some $d^{⁎}\in \{5,6,7\}$.     

螺栓连接大变形梁非线性振动特性的实验研究

柔性机械臂、大型可展开天线等机械结构的动作精度受运动过程中大变形几何非线性和连接处接触非线性的影响十分显著。以含螺栓连接结构的大变形梁作为研究对象，针对动力学建模和振动特性开展了实验研究，通过数值计算验证了实验发现的非线性振动特性。搭建了含螺栓连接柔性大变形梁的实验台架，开展了敲击和正弦激振的实验测试。实验结果表明，螺栓连接的柔性梁较连续梁的（无螺栓连接）模态频率降低，阻尼增加，反映出随着激励能量增大，模态频率降低的非线性模态特征。改变螺栓连接位置会显著影响结构的模态频率，其变化规律可由求解线性矩阵特征值定性反映。     

基于混合优化算法的关节臂式测量机参数标定

为进一步提高关节臂式坐标测量机等高机动性精密测量设备的测量精度,使用D-H矩阵法建立其关节坐标转换数学模型并据此推导出参数误差模型.针对非线性多参数标定问题,通过变换分析消除了最小二乘法求解时矩阵中的冗余参数,降低了计算的复杂性.设定判定准则并实现最小二乘法和模拟退火算法的混合,提出了一种基于混合优化算法的参数标定方法,解决了LM算法的初值设定和SA算法的搜索效率逐步降低的问题.实验结果表明:关节臂式测量机参数经混合优化算法标定后,参数的误差范围有了显著的缩小,单点重复性误差的平均值减小了1.746 mm,长度误差的平均值减小了0.941 mm,测量误差得到了进一步的抑制.     

Synthesis and investigation of structural,optical, magnetic,and biocompatibility properties of nanoferrites AFeO2

Sol-gel method has been used for the synthesis of biocompatible superparamagnetic nanoferrites of AFeO₂ (A = Li, Na, K, Ca). Structural study of the nanoferrites reveals that LiFeO₂ exhibits cubic phase on the other hand NaFeO₂, KFeO₂, CaFeO₂ nanoparticles possess orthorhombic phase. Transmission electron microscopy (TEM) suggests that synthesized nanoferrites are nano-sized with spherical morphology. Optical properties confirm that nanoferrites emit and absorb light in a visible range of the electromagnetic spectrum. International Commission on Illumination (CIE) study discloses that the nanoparticles can be used to produce light of various colors. Magnetic study reveals that the nanoferrites exhibit superparamagnetic nature with high values of saturation magnetization 40.26 emu/g, 41.69 emu/g, 57.16 emu/g, and 43.66 emu/g, respectively for LiFeO_2, NaFeO₂, KFeO₂, and CaFeO₂. Biocompatibility study of the nanoferrites has been performed using Sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The reason behind the observed properties and behavior has been discussed.     

PI-强LU富足半群

通过对C-L^U-富足半群的刻画,给出了PI-强L^U富足半群的结构定理。     

高陡横坡段桩柱式桥梁双桩基础受力分析

基于高陡横坡段桩柱式桥梁双桩基础承载特性,提出了一种改进有限杆单元计算分析方法.分析了高陡横坡段桩柱式桥梁双桩基础承载机理及受力特性,建立了双桩基础计算分析模型.其次,根据前、后桩与边坡相对位置关系,给出了后桩所受剩余下滑力与前桩所受土压力的比例关系.在传统有限杆单元分析方法基础上,结合陡坡桩受力特征,导得了考虑桩土共同作用与"P-Δ"效应的单元刚度矩阵修正方法,并在此基础上编制了适用于高陡横坡段桩柱式桥梁双桩基础的有限杆单元分析MATLAB计算程序.采用室内模型试验对本文计算方法进行验证,给出了适用于陡坡段桥梁桩基的设计流程图.研究结果表明:本文理论计算值与模型试验实测结果吻合良好,表明本文计算方法正确可行,可为同类工程设计提供参考.